Phospho-N-WASP (Tyr256) Antibody - #AF7032

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製品: Phospho-N-WASP (Tyr256) Antibody
カタログ: AF7032
タンパク質の説明: Rabbit polyclonal antibody to Phospho-N-WASP (Tyr256)
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 54kDa; 55kD(Calculated).
ユニプロット: O00401
RRID: AB_2843472

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-N-WASP (Tyr256) Antibody detects endogenous levels of N-WASP only when phosphorylated at Tyr256.
RRID:
AB_2843472
引用形式: Affinity Biosciences Cat# AF7032, RRID:AB_2843472.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DKFZp779G0847; MGC48327; N-WASP; Neural Wiskott Aldrich syndrome protein; Neural Wiskott-Aldrich syndrome protein; NWASP; Wasl; WASL_HUMAN; WASPB; Wiskott Aldrich syndrome gene like; Wiskott Aldrich syndrome gene like protein; Wiskott Aldrich syndrome like; WiskottAldrich syndrome like;

免疫原

免疫原:

A synthesized peptide derived from human N-WASP around the phosphorylation site of Tyr256.

Uniprot:

O00401(WASL_HUMAN) >>Visit HPA database.

遺伝子(ID):
WASL(8976)
タンパク質配列:
MSSVQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHNSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPSVAVPPPPPNRMYPPPPPALPSSAPSGPPPPPPSVLGVGPVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTTAGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVADGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDEDDEEDFEDDDEWED

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
90
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization. Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression (By similarity). Plays a role in dendrite spine morphogenesis (By similarity). Decreasing levels of DNMBP (using antisense RNA) alters apical junction morphology in cultured enterocytes, junctions curve instead of being nearly linear.

PTMs:

Phosphorylation at Ser-242, Tyr-256, Ser-484 and Ser-485 enhances actin polymerization activity.

細胞の位置付け:

Cytoplasm>Cytoskeleton. Nucleus. Cytoplasm.
Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated (By similarity). Exported from the nucleus by an nuclear export signal (NES)-dependent mechanism to the cytoplasm (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

参考文献

1). Novel long noncoding RNA LINC02820 augments TNF signaling pathway to remodel cytoskeleton and potentiate metastasis in esophageal squamous cell carcinoma. Cancer Gene Therapy, 2022 (PubMed: 36357564) [IF=4.8]

Application: WB    Species: Mouse    Sample:

Fig. 3 LINC02820 promotes metastasis in ESCC via cytoskeletal remodeling, not EMT. A Analysis of the migration and invasion ability of K180 cells transfected with SiLINC02820 or siNC (scale bar, 100 μm). B Analysis of the migration and invasion ability of K30 cells transfected with OE- LINC02820 or Vector (scale bar, 100 μm). C The migration and invasion ability of K180 cells transfected with LINC02820 suppression plasmid (sg-3 and sg-4) or control plasmid (Guide) (scale bar, 100 μm). D, E K30 Vector and K30 OE-LINC02820 cell lines were injected in mice footpads and the popliteal lymph node of nude mice was shown, and the metastasis of the popliteal lymph node. F The protein levels of EMT markers when LINC02820 are inhibited or increased. G The levels of N-WASP, p-N-WASP, and Cortactin about invasive pseudopodia. H Inhibition of LINC02820 reduced the F-actin in K180 and K410 cells (scale bar, 10 μm). I Overexpression of LINC02820 increased the F-actin in K30 and EC109 cells (scale bar, 10 μm).

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