製品: V-ATPase H Antibody
カタログ: AF9227
タンパク質の説明: Rabbit polyclonal antibody to V-ATPase H
アプリケーション: WB IHC
反応性: Human, Mouse
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 55kDa; 56kD(Calculated).
ユニプロット: Q9UI12
RRID: AB_2843417

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
V-ATPase H Antibody detects endogenous levels of total V-ATPase H.
RRID:
AB_2843417
引用形式: Affinity Biosciences Cat# AF9227, RRID:AB_2843417.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Atp6v1h; ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H; CGI 11; CGI11; EC 3.6.3.14; MSTP042; NBP1; Nef binding protein 1; Nef-binding protein 1; Protein VMA13 homolog; SFD; SFDalpha; SFDbeta; V ATPase subunit H; V-ATPase 50/57 kDa subunits; V-ATPase subunit H; V-type proton ATPase subunit H; Vacuolar ATP synthase subunit H; Vacuolar proton pump subunit H; Vacuolar proton pump subunit SFD; VATH_HUMAN; VMA13;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q9UI12 VATH_HUMAN:

Widely expressed.

タンパク質配列:
MTKMDIRGAVDAAVPTNIIAAKAAEVRANKVNWQSYLQGQMISAEDCEFIQRFEMKRSPEEKQEMLQTEGSQCAKTFINLMTHICKEQTVQYILTMVDDMLQENHQRVSIFFDYARCSKNTAWPYFLPMLNRQDPFTVHMAARIIAKLAAWGKELMEGSDLNYYFNWIKTQLSSQKLRGSGVAVETGTVSSSDSSQYVQCVAGCLQLMLRVNEYRFAWVEADGVNCIMGVLSNKCGFQLQYQMIFSIWLLAFSPQMCEHLRRYNIIPVLSDILQESVKEKVTRIILAAFRNFLEKSTERETRQEYALAMIQCKVLKQLENLEQQKYDDEDISEDIKFLLEKLGESVQDLSSFDEYSSELKSGRLEWSPVHKSEKFWRENAVRLNEKNYELLKILTKLLEVSDDPQVLAVAAHDVGEYVRHYPRGKRVIEQLGGKQLVMNHMHHEDQQVRYNALLAVQKLMVHNWEYLGKQLQSEQPQTAAARS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UI12 基板として

Site PTM Type Enzyme
T16 Phosphorylation
K22 Ubiquitination
K62 Ubiquitination
K176 Ubiquitination
K313 Ubiquitination
K316 Ubiquitination
K325 Ubiquitination
K341 Ubiquitination
S351 Phosphorylation
K360 Ubiquitination
S367 Phosphorylation
K371 Ubiquitination
K374 Ubiquitination
K386 Ubiquitination
Y388 Phosphorylation
K396 Ubiquitination
Y466 Phosphorylation
K469 Ubiquitination
S483 Phosphorylation

研究背景

機能:

Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes.

組織特異性:

Widely expressed.

サブユニット構造:

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). Interacts with AP2M1. Interacts with TM9SF4 in colon cancer cells.

(Microbial infection) Interacts with HIV-1 Nef protein.

タンパク質ファミリー:

Belongs to the V-ATPase H subunit family.

研究領域

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

参考文献

1). Bioengineered microglia-targeted exosomes facilitate Aβ clearance via enhancing activity of microglial lysosome for promoting cognitive recovery in Alzheimer's disease. Biomaterials Advances (PubMed: 35929330)

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