TCFL5 Antibody - #AF9211

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製品: TCFL5 Antibody
カタログ: AF9211
タンパク質の説明: Rabbit polyclonal antibody to TCFL5
アプリケーション: WB IF/ICC
Cited expt.: IF/ICC
反応性: Human, Mouse
予測: Pig, Sheep, Rabbit, Dog, Chicken
分子量: 48kDa(Observed); 53kD(Calculated).
ユニプロット: Q9UL49
RRID: AB_2843401

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#AF9211-BP

TCFL5 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Sheep(100%), Rabbit(91%), Dog(100%), Chicken(91%)
クローナリティ:
Polyclonal
特異性:
TCFL5 Antibody detects endogenous levels of total TCFL5.
RRID:
AB_2843401
引用形式: Affinity Biosciences Cat# AF9211, RRID:AB_2843401.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

bHLHe82; CHA; Cha transcription factor; E2BP-1; E2BP1; Figlb; HPV-16 E2-binding protein 1; MGC46135; TCFL 5; TCFL5; TCFL5_HUMAN; transcription factor-like 5 (basic helix-loop-helix); Transcription factor-like 5 protein;

免疫原

免疫原:

A synthesized peptide derived from human TCFL5, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9UL49(TCFL5_HUMAN) >>Visit HPA database.

遺伝子(ID):
TCFL5(10732)
発現特異性:
Q9UL49 TCFL5_HUMAN:

Isoform 3 is testis specific. Isoform 2 is pancreas specific.

タンパク質配列:
MSGPGPREPPPEAGAAGGEAAVEGAGGGDAALGEPGLSFTTTDLSLVEMTEVEYTQLQHILCSHMEAAADGELETRLNSALLAAAGPGAGAGGFAAGGQGGAAPVYPVLCPSALAADAPCLGHIDFQELRMMLLSEAGAAEKTSGGGDGARARADGAAKEGAGAAAAAAGPDGAPEARAKPAVRVRLEDRFNSIPAEPPPAPRGPEPPEPGGALNNLVTLIRHPSELMNVPLQQQNKCTALVKNKTAATTTALQFTYPLFTTNACSTSGNSNLSQTQSSSNSCSVLEAAKHQDIGLPRAFSFCYQQEIESTKQTLGSRNKVLPEQVWIKVGEAALCKQALKRNRSRMRQLDTNVERRALGEIQNVGEGATATQGAWQSSESSQANLGEQAQSGPQGGRSQRRERHNRMERDRRRRIRICCDELNLLVPFCNAETDKATTLQWTTAFLKYIQERHGDSLKKEFESVFCGKTGRRLKLTRPDSLVTCPAQGSLQSSPSMEIK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Chicken
91
Rabbit
91
Horse
0
Bovine
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Putative transcription factor. Isoform 3 may play a role in early spermatogenesis.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Isoform 3 is testis specific. Isoform 2 is pancreas specific.

参考文献

1). Single cell epigenomic and transcriptomic analysis uncovers potential transcription factors regulating mitotic/meiotic switch. Cell death & disease, 2023 (PubMed: 36797258) [IF=8.1]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 5: Identification of TFs in female germ cells associated with mitotic/meiotic switch. A Workflow of the selected framework of potential core TFs in meiotic initiation. B Final acquired core TFs of meiotic initiation. C Dot plot shows the expression trend of final acquired potential core TFs. The upper panel shows Pearson’s correlation between core TFs and Stra8. D Sankey diagram of TFs interaction network; Blue represents strong interactions, black represents weak interactions. E Function enrichment network of TCFL5 potential bound genes. F The epigenetic characteristics of Tcfl5; Volcano plot shows the gene activity score of Stra8 in cell subtypes. G TCFL5 immunolocalization on chromosomes of germ cells at different stages of meiotic prophase I; bar = 1 μm, n ≥ 11; Red shows SYCP3, green showsTCFL5, and blue shows nuclei; H The barplot showing the expression level of TCFL5 protein at different stages of meiotic prophase I. ** means p value 

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