RHOBTB3 Antibody - #AF9183

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製品: RHOBTB3 Antibody
カタログ: AF9183
タンパク質の説明: Rabbit polyclonal antibody to RHOBTB3
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Rat
予測: Pig, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 70kDa; 69kD(Calculated).
ユニプロット: O94955
RRID: AB_2843373

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説明書
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プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
予測:
Pig(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
RHOBTB3 Antibody detects endogenous levels of total RHOBTB3.
RRID:
AB_2843373
引用形式: Affinity Biosciences Cat# AF9183, RRID:AB_2843373.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

KIAA0878; Rho related BTB domain containing 3; Rho related BTB domain containing protein 3; RHOBTB 3;

免疫原

免疫原:

A synthesized peptide derived from human RHOBTB3, corresponding to a region within the internal amino acids.

Uniprot:

O94955(RHBT3_HUMAN) >>Visit HPA database.

遺伝子(ID):
RHOBTB3(22836)
発現特異性:
O94955 RHBT3_HUMAN:

Ubiquitous. Highly expressed in neural and cardiac tissues, pancreas, placenta and testis.

タンパク質配列:
MSIHIVALGNEGDTFHQDNRPSGLIRTYLGRSPLVSGDESSLLLNAASTVARPVFTEYQASAFGNVKLVVHDCPVWDIFDSDWYTSRNLIGGADIIVIKYNVNDKFSFHEVKDNYIPVIKRALNSVPVIIAAVGTRQNEELPCTCPLCTSDRGSCVSTTEGIQLAKELGATYLELHSLDDFYIGKYFGGVLEYFMIQALNQKTSEKMKKRKMSNSFHGIRPPQLEQPEKMPVLKAEASHYNSDLNNLLFCCQCVDVVFYNPNLKKVVEAHKIVLCAVSHVFMLLFNVKSPTDIQDSSIIRTTQDLFAINRDTAFPGASHESSGNPPLRVIVKDALFCSCLSDILRFIYSGAFQWEELEEDIRKKLKDSGDVSNVIEKVKCILKTPGKINCLRNCKTYQARKPLWFYNTSLKFFLNKPMLADVVFEIQGTTVPAHRAILVARCEVMAAMFNGNYMEAKSVLIPVYGVSKETFLSFLEYLYTDSCCPAGIFQAMCLLICAEMYQVSRLQHICELFIITQLQSMPSRELASMNLDIVDLLKKAKFHHSDCLSTWLLHFIATNYLIFSQKPEFQDLSVEERSFVEKHRWPSNMYLKQLAEYRKYIHSRKCRCLVM

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
88
Bovine
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Rab9-regulated ATPase required for endosome to Golgi transport. Involved in transport vesicle docking at the Golgi complex, possibly by participating in release M6PRBP1/TIP47 from vesicles to permit their efficient docking and fusion at the Golgi. Specifically binds Rab9, but not other Rab proteins. Has low intrinsic ATPase activity due to autoinhibition, which is relieved by Rab9.

細胞の位置付け:

Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous. Highly expressed in neural and cardiac tissues, pancreas, placenta and testis.

タンパク質ファミリー:

Although predicted to be a GTP-binding protein because of the presence of a Rho-like region, binds and hydrolyzes ATP. In contrast to Rho-like proteins, the conserved Asp residue in position 138 in the G4 region is replaced by an Asn, decreasing the ability to bind GTP.

参考文献

1). MicroRNA-142-3p promotes renal cell carcinoma progression by targeting RhoBTB3 to regulate HIF-1 signaling and GGT/GSH pathways. Scientific reports, 2023 (PubMed: 37045834) [IF=3.8]

Application: WB    Species: Human    Sample: 786-O cells

Figure 4. miR-142-3p regulates RCC migration and invasion via hypoxia and GSH pathways by targeting RhoBTB3. 786-O cells were transfected with miR-control, miR-142-3p-mimics, and, miR-142-3p-inhibitors. Protein expression of RhoBTB3, hypoxia, and GSH pathway-related biomarkers, including HIF1A, VEGFA, and GGT1 using Western blot (A) (the original blots/gels are presented in Supplementary Fig. 3, the samples derive from the same experiment and that gels/blots were processed in parallel). The mRNA and protein expression of RhoBTB3, HIF1A, VEGFA, and GGT1 in 786-O cells was determined using qRT-PCR and Western blot, respectively (B,C). Expression of GGT1 differed significantly between RCC and the normal adjacent tissues (enhanced in KIRC, D, P 

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