Rab 34 Antibody - #AF9174

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製品: Rab 34 Antibody
カタログ: AF9174
タンパク質の説明: Rabbit polyclonal antibody to Rab 34
アプリケーション: WB IHC IF/ICC
Cited expt.: IHC
反応性: Human, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 29kDa; 29kD(Calculated).
ユニプロット: Q9BZG1
RRID: AB_2843364

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 100ul $280 在庫あり
 200ul $350 在庫あり

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(89%)
クローナリティ:
Polyclonal
特異性:
Rab 34 Antibody detects endogenous levels of total Rab 34.
RRID:
AB_2843364
引用形式: Affinity Biosciences Cat# AF9174, RRID:AB_2843364.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Rab-39; RAB34; RAB34, member RAS oncogene family; RAB34_HUMAN; RAB39; RAH; Ras-related protein Rab-34; Ras-related protein Rab-39; Ras-related protein Rah;

免疫原

免疫原:

A synthesized peptide derived from human Rab 34, corresponding to a region within the internal amino acids.

Uniprot:

Q9BZG1(RAB34_HUMAN) >>Visit HPA database.

遺伝子(ID):
RAB34(83871)
タンパク質配列:
MNILAPVRRDRVLAELPQCLRKEAALHGHKDFHPRVTCACQEHRTGTVGFKISKVIVVGDLSVGKTCLINRFCKDTFDKNYKATIGVDFEMERFEVLGIPFSLQLWDTAGQERFKCIASTYYRGAQAIIIVFNLNDVASLEHTKQWLADALKENDPSSVLLFLVGSKKDLSTPAQYALMEKDALQVAQEMKAEYWAVSSLTGENVREFFFRVAALTFEANVLAELEKSGARRIGDVVRINSDDSNLYLTASKKKPTCCP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
89
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Protein transport. Involved in the redistribution of lysosomes to the peri-Golgi region (By similarity). Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Acts also as a positive regulator of hedgehog signaling and regulates ciliary function (By similarity).

細胞の位置付け:

Cytoplasm. Golgi apparatus. Cytoplasmic vesicle>Phagosome. Cytoplasmic vesicle>Phagosome membrane>Lipid-anchor>Cytoplasmic side. Cell projection>Cilium.
Note: Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the small GTPase superfamily. Rab family.

参考文献

1). The Role of RAB GTPases and Its Potential in Predicting Immunotherapy Response and Prognosis in Colorectal Cancer. Frontiers in Genetics, 2022 (PubMed: 35154286) [IF=3.7]

Application: IHC    Species: Human    Sample: HCT16 cells and SW480 cells

FIGURE 8 Overexpression of RAB34 increased PD-L1 and PD-L2 expression of CRC. (A) Immunohistochemical staining of patients with colorectal cancer. (B) Overexpression of RAB34 analyzed the protein levels of PDL-1 and PD-L2 in the HCT16 cells and SW480 cells. (C) Overexpression of RAB34 analyzed the migration and invasion in the HCT16 cells and SW480 cells. * p < .05, ** p < .01, *** p < .001.

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