製品: POLR3B Antibody
カタログ: AF9165
タンパク質の説明: Rabbit polyclonal antibody to POLR3B
アプリケーション: WB IF/ICC
反応性: Human, Rat
予測: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 128kDa; 128kD(Calculated).
ユニプロット: Q9NW08
RRID: AB_2843355

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
予測:
Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(92%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
POLR3B Antibody detects endogenous levels of total POLR3B.
RRID:
AB_2843355
引用形式: Affinity Biosciences Cat# AF9165, RRID:AB_2843355.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

C128; DNA directed RNA polymerase III 127.6 kDa polypeptide; DNA directed RNA polymerase III subunit B; DNA-directed RNA polymerase III 127.6 kDa polypeptide; DNA-directed RNA polymerase III subunit B; DNA-directed RNA polymerase III subunit RPC2; FLJ10388; polr3b; Polymerase (RNA) III (DNA directed) polypeptide B; RNA polymerase III subunit C2; RNA polymerase III subunit RPC2; RPC2; RPC2_HUMAN;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MDVLAEEFGNLTPEQLAAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGAVGASVTSSTHEKKSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIVKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVNTFRLMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIEFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHGYNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLSKYNE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Dog
92
Zebrafish
92
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NW08 基板として

Site PTM Type Enzyme
T12 Phosphorylation
K64 Ubiquitination
K157 Ubiquitination
K172 Ubiquitination
K186 Ubiquitination
K196 Ubiquitination
S202 Phosphorylation
S206 Phosphorylation
K210 Ubiquitination
K290 Ubiquitination
T304 Phosphorylation
K324 Ubiquitination
K394 Ubiquitination
K404 Ubiquitination
K601 Ubiquitination
K609 Ubiquitination
S680 Phosphorylation
K691 Ubiquitination
T707 Phosphorylation
Y710 Phosphorylation
Y714 Phosphorylation
T722 Phosphorylation
K723 Ubiquitination
S816 Phosphorylation
K820 Ubiquitination
K824 Ubiquitination
K830 Ubiquitination
K852 Ubiquitination
K859 Methylation
K859 Ubiquitination
S872 Phosphorylation
S873 Phosphorylation
K896 Ubiquitination
K950 Ubiquitination
K968 Ubiquitination
K970 Ubiquitination
K1013 Methylation
K1019 Ubiquitination
T1036 Phosphorylation

研究背景

機能:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.

タンパク質ファミリー:

Belongs to the RNA polymerase beta chain family.

研究領域

· Genetic Information Processing > Transcription > RNA polymerase.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

Restrictive clause

 

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