Peropsin Antibody - #AF9153

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製品: Peropsin Antibody
カタログ: AF9153
タンパク質の説明: Rabbit polyclonal antibody to Peropsin
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human
予測: Pig, Bovine, Sheep, Dog, Chicken
分子量: 37kDa; 37kD(Calculated).
ユニプロット: O14718
RRID: AB_2843343

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(100%), Bovine(90%), Sheep(90%), Dog(90%), Chicken(80%)
クローナリティ:
Polyclonal
特異性:
Peropsin Antibody detects endogenous levels of total Peropsin.
RRID:
AB_2843343
引用形式: Affinity Biosciences Cat# AF9153, RRID:AB_2843343.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

OPSX; OPSX_HUMAN; Retinal pigment epithelium derived rhodopsin homolog; Rrh; Visual pigment like receptor peropsin.; Visual pigment-like receptor peropsin;

免疫原

免疫原:

A synthesized peptide derived from human Peropsin, corresponding to a region within the internal amino acids.

Uniprot:

O14718(OPSX_HUMAN) >>Visit HPA database.

遺伝子(ID):
RRH(10692)
発現特異性:
O14718 OPSX_HUMAN:

Found only in the eye, where it is localized to the retinal pigment epithelium (RPE). In the RPE, it is localized to the microvilli that surround the photoreceptor outer segments.

タンパク質配列:
MLRNNLGNSSDSKNEDGSVFSQTEHNIVATYLIMAGMISIISNIIVLGIFIKYKELRTPTNAIIINLAVTDIGVSSIGYPMSAASDLYGSWKFGYAGCQVYAGLNIFFGMASIGLLTVVAVDRYLTICLPDVGRRMTTNTYIGLILGAWINGLFWALMPIIGWASYAPDPTGATCTINWRKNDRSFVSYTMTVIAINFIVPLTVMFYCYYHVTLSIKHHTTSDCTESLNRDWSDQIDVTKMSVIMICMFLVAWSPYSIVCLWASFGDPKKIPPPMAIIAPLFAKSSTFYNPCIYVVANKKFRRAMLAMFKCQTHQTMPVTSILPMDVSQNPLASGRI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
90
Sheep
90
Dog
90
Chicken
80
Horse
70
Zebrafish
70
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

May play a role in rpe physiology either by detecting light directly or by monitoring the concentration of retinoids or other photoreceptor-derived compounds.

細胞の位置付け:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Found only in the eye, where it is localized to the retinal pigment epithelium (RPE). In the RPE, it is localized to the microvilli that surround the photoreceptor outer segments.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

参考文献

1). Exposure to blue light stimulates the proangiogenic capability of exosomes derived from human umbilical cord mesenchymal stem cells. Stem Cell Research & Therapy, 2019 (PubMed: 31779691) [IF=7.5]

Application: WB    Species: Human    Sample: mesenchymal stem cells

Fig. 1 Characterization of MSC photoreceptors for blue/red light irradiation. a Schema of the LED photosystem. b Real-time PCR screening of the basal and stimulated expression of opsins as indicated (OPN4 constitutional expression level was selected as the control group, fold-change = 1). c Proteins were extracted from P2, P4, and P6 passages of MSCs and subjected to immunoblotting. d Proteins were extracted from P4 passage of MSCs with blue/red light illumination and subjected to immunoblotting. e Immunofluorescence staining of the indicated photoreceptors in MSCs

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