Nav1.7 Antibody - #AF9120
製品: | Nav1.7 Antibody |
カタログ: | AF9120 |
タンパク質の説明: | Rabbit polyclonal antibody to Nav1.7 |
アプリケーション: | WB |
反応性: | Human, Mouse |
予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
分子量: | 220kDa; 226kD(Calculated). |
ユニプロット: | Q15858 |
RRID: | AB_2843310 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF9120, RRID:AB_2843310.
折りたたみ/展開
ETHA; GEFSP7; hNE Na; hNE-Na; hNENa; NE NA; NENA; Neuroendocrine sodium channel; Peripheral sodium channel 1; PN1; Scn9a; SCN9A_HUMAN; Sodium channel protein type 9 subunit alpha; Sodium channel protein type IX subunit alpha; Sodium channel voltage gated type IX alpha; Sodium channel voltage gated type IX alpha polypeptide; Sodium channel voltage gated type IX alpha subunit; Voltage gated sodium channel alpha subunit Nav1.7; Voltage gated sodium channel subunit alpha Nav1; Voltage-gated sodium channel subunit alpha Nav1.7;
免疫原
Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root ganglion.
- Q15858 SCN9A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAMLPPPGPQSFVHFTKQSLALIEQRIAERKSKEPKEEKKDDDEEAPKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKTIFRFNATPALYMLSPFSPLRRISIKILVHSLFSMLIMCTILTNCIFMTMNNPPDWTKNVEYTFTGIYTFESLVKILARGFCVGEFTFLRDPWNWLDFVVIVFAYLTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCFRNSLENNETLESIMNTLESEEDFRKYFYYLEGSKDALLCGFSTDSGQCPEGYTCVKIGRNPDYGYTSFDTFSWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYEEQNQANIEEAKQKELEFQQMLDRLKKEQEEAEAIAAAAAEYTSIRRSRIMGLSESSSETSKLSSKSAKERRNRRKKKNQKKLSSGEEKGDAEKLSKSESEDSIRRKSFHLGVEGHRRAHEKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDIGSETEFADDEHSIFGDNESRRGSLFVPHRPQERRSSNISQASRSPPMLPVNGKMHSAVDCNGVVSLVDGRSALMLPNGQLLPEVIIDKATSDDSGTTNQIHKKRRCSSYLLSEDMLNDPNLRQRAMSRASILTNTVEELEESRQKCPPWWYRFAHKFLIWNCSPYWIKFKKCIYFIVMDPFVDLAITICIVLNTLFMAMEHHPMTEEFKNVLAIGNLVFTGIFAAEMVLKLIAMDPYEYFQVGWNIFDSLIVTLSLVELFLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINDDCTLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVYMMVMVIGNLVVLNLFLALLLSSFSSDNLTAIEEDPDANNLQIAVTRIKKGINYVKQTLREFILKAFSKKPKISREIRQAEDLNTKKENYISNHTLAEMSKGHNFLKEKDKISGFGSSVDKHLMEDSDGQSFIHNPSLTVTVPIAPGESDLENMNAEELSSDSDSEYSKVRLNRSSSSECSTVDNPLPGEGEEAEAEPMNSDEPEACFTDGCVWRFSCCQVNIESGKGKIWWNIRKTCYKIVEHSWFESFIVLMILLSSGALAFEDIYIERKKTIKIILEYADKIFTYIFILEMLLKWIAYGYKTYFTNAWCWLDFLIVDVSLVTLVANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYECINTTDGSRFPASQVPNRSECFALMNVSQNVRWKNLKVNFDNVGLGYLSLLQVATFKGWTIIMYAAVDSVNVDKQPKYEYSLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKIQGCIFDLVTNQAFDISIMVLICLNMVTMMVEKEGQSQHMTEVLYWINVVFIILFTGECVLKLISLRHYYFTVGWNIFDFVVVIISIVGMFLADLIETYFVSPTLFRVIRLARIGRILRLVKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEDGINDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPKKVHPGSSVEGDCGNPSVGIFYFVSYIIISFLVVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVWEKFDPDATQFIEFSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEDVSATVIQRAYRRYRLRQNVKNISSIYIKDGDRDDDLLNKKDMAFDNVNENSSPEKTDATSSTTSPPSYDSVTKPDKEKYEQDRTEKEDKGKDSKESKK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15858 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K36 | Ubiquitination | Uniprot | |
K39 | Ubiquitination | Uniprot | |
S113 | Phosphorylation | Uniprot | |
S453 | Phosphorylation | Uniprot | |
S459 | Phosphorylation | Uniprot | |
K483 | Acetylation | Uniprot | |
K494 | Acetylation | Uniprot | |
S530 | Phosphorylation | Uniprot | |
T531 | Phosphorylation | Uniprot | |
S535 | Phosphorylation | Uniprot | |
S538 | Phosphorylation | Uniprot | |
S542 | Phosphorylation | Uniprot | |
S545 | Phosphorylation | Uniprot | |
S691 | Phosphorylation | Uniprot | |
K1034 | Acetylation | Uniprot | |
S1164 | Phosphorylation | Uniprot | |
T1234 | Phosphorylation | Uniprot | |
Y1413 | Phosphorylation | Uniprot | |
S1418 | Phosphorylation | Uniprot | |
K1480 | Acetylation | Uniprot | |
Y1481 | Phosphorylation | Uniprot | |
Y1482 | Phosphorylation | Uniprot | |
S1490 | Phosphorylation | Uniprot | |
Y1658 | Phosphorylation | Uniprot | |
Y1668 | Phosphorylation | Uniprot | |
S1873 | Phosphorylation | Uniprot | |
Y1877 | Phosphorylation | Uniprot | |
T1881 | Phosphorylation | Uniprot | |
T1882 | Phosphorylation | Uniprot | |
T1883 | Phosphorylation | Uniprot | |
S1950 | Phosphorylation | Uniprot | |
K1981 | Acetylation | Uniprot |
研究背景
Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain.
Phosphorylation at Ser-1490 by PKC in a highly conserved cytoplasmic loop increases peak sodium currents.
Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4.
Cell membrane>Multi-pass membrane protein. Cell projection>Neuron projection.
Note: In neurite terminals.
Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root ganglion.
The sodium channel complex consists of a large, channel-forming alpha subunit (SCN9A) regulated by one or more beta subunits (SCN1B, SCN2B, SCN3B and SCN4B). SCN1B and SCN3B are non-covalently associated with SCN2A. SCN2B and SCN4B are disulfide-linked to SCN2A. Interacts with NEDD4 and NEDD4L (By similarity). Interacts with the scorpion alpha-toxin CvIV4. Interacts with the conotoxin GVIIJ. Interacts with the spider huwentoxin-IV (through the extracellular loop S3-S4 of repeat II). Interacts with the spider protoxin-II (through the extracellular loop S3-S4 of repeats II and IV). Interacts with the spider beta/delta-theraphotoxin-Pre1a.
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1, S2, S3, S5, S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.7/SCN9A subfamily.
研究領域
· Organismal Systems > Sensory system > Taste transduction.
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