MINK1 Antibody - #AF9112
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF9112, RRID:AB_2843302.
折りたたみ／展開
B55; GCK family kinase MiNK; hMINK; Map4k6; MAPK/ERK kinase kinase kinase 6; MEK (MAP/ERK) kinase kinase 6; MEK kinase kinase 6; MEKKK6; MGC21111; MINK 1; MINK; MINK1; Misshapen like kinase 1; Misshapen NIKs Related kinase; Misshapen/NIK related kinase; Mitogen activated protein kinase kinase kinase kinase 6; RP23-122P1.6; Ysk2; ZC3;
免疫原
Expressed in the brain, isoform 2 is more abundant than isoform 1. Isoform 3 is ubiquitously expressed. Isoform 1 is most abundant in the skeletal muscle. Isoform 4 is ubiquitously expressed with relative high levels in brain, skeletal muscle, pancreas and testis.
- Q8N4C8 MINK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQLLPGDRKPLYHYGRGMNPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAIPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGVSSKPDSSPVLSPGNKAKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDDEEEGEGGPAEGSRDTPGGRSDGDTDSVSTMVVHDVEEITGTQPPYGGGTMVVQRTPEEERNLLHADSNGYTNLPDVVQPSHSPTENSKGQSPPSKDGSGDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDSIPITALVGGEGTRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8N4C8 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S9 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| T59 | Phosphorylation | Uniprot | |
| Y86 | Phosphorylation | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Q8N4C8 (MINK1) | Uniprot |
| T191 | Phosphorylation | Q8N4C8 (MINK1) | Uniprot |
| Y193 | Phosphorylation | Q8N4C8 (MINK1) | Uniprot |
| T280 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| K303 | Ubiquitination | Uniprot | |
| Y321 | Phosphorylation | Uniprot | |
| Y323 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| Y475 | Phosphorylation | Uniprot | |
| K477 | Ubiquitination | Uniprot | |
| S478 | Phosphorylation | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| Y505 | Phosphorylation | Uniprot | |
| Y507 | Phosphorylation | Uniprot | |
| R509 | Methylation | Uniprot | |
| S535 | Phosphorylation | Uniprot | |
| S563 | Phosphorylation | Uniprot | |
| T565 | Phosphorylation | Uniprot | |
| S581 | Phosphorylation | Uniprot | |
| K590 | Acetylation | Uniprot | |
| K590 | Methylation | Uniprot | |
| Y592 | Phosphorylation | Uniprot | |
| S601 | Phosphorylation | Uniprot | |
| T629 | Phosphorylation | Uniprot | |
| S641 | Phosphorylation | Uniprot | |
| T644 | Phosphorylation | Uniprot | |
| S673 | Phosphorylation | Uniprot | |
| S674 | Phosphorylation | Uniprot | |
| S699 | Phosphorylation | Uniprot | |
| S701 | Phosphorylation | Uniprot | |
| Y706 | Phosphorylation | Uniprot | |
| S732 | Phosphorylation | Uniprot | |
| S733 | Phosphorylation | Uniprot | |
| S740 | Phosphorylation | Uniprot | |
| S747 | Phosphorylation | Uniprot | |
| S749 | Phosphorylation | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| S763 | Phosphorylation | Uniprot | |
| S772 | Phosphorylation | Uniprot | |
| S773 | Phosphorylation | Uniprot | |
| S777 | Phosphorylation | Uniprot | |
| S778 | Phosphorylation | Uniprot | |
| S782 | Phosphorylation | Uniprot | |
| T809 | Phosphorylation | Uniprot | |
| S903 | Phosphorylation | Uniprot | |
| Y906 | Phosphorylation | Uniprot | |
| T907 | Phosphorylation | Uniprot | |
| S916 | Phosphorylation | Uniprot | |
| S918 | Phosphorylation | Uniprot | |
| T920 | Phosphorylation | Uniprot | |
| S927 | Phosphorylation | Uniprot | |
| K931 | Ubiquitination | Uniprot | |
| Y937 | Phosphorylation | Uniprot | |
| S939 | Phosphorylation | Uniprot | |
| Y960 | Phosphorylation | Uniprot | |
| Y987 | Phosphorylation | Uniprot | |
| S993 | Phosphorylation | Uniprot | |
| T1008 | Phosphorylation | Uniprot | |
| S1088 | Phosphorylation | Uniprot | |
| K1103 | Ubiquitination | Uniprot | |
| K1146 | Ubiquitination | Uniprot | |
| K1150 | Ubiquitination | Uniprot | |
| K1291 | Ubiquitination | Uniprot | |
| K1297 | Ubiquitination | Uniprot | |
| K1305 | Ubiquitination | Uniprot | |
| S1317 | Phosphorylation | Uniprot | |
| Y1320 | Phosphorylation | Uniprot | |
| T1323 | Phosphorylation | Uniprot |
研究背景
Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1.
Isoform 4 can activate the JNK pathway. Involved in the regulation of actin cytoskeleton reorganization, cell-matrix adhesion, cell-cell adhesion and cell migration.
Autophosphorylated.
Cytoplasm. Cell junction>Synapse>Postsynaptic density. Cell projection>Axon. Cell projection>Dendrite.
Golgi apparatus.
Expressed in the brain, isoform 2 is more abundant than isoform 1. Isoform 3 is ubiquitously expressed. Isoform 1 is most abundant in the skeletal muscle. Isoform 4 is ubiquitously expressed with relative high levels in brain, skeletal muscle, pancreas and testis.
Interacts with TANC1 (By similarity). Interacts with RAP2A. Isoform 4 interacts with NCK1.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
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