TRAPPC5 Antibody - #DF9965

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製品: TRAPPC5 Antibody
カタログ: DF9965
タンパク質の説明: Rabbit polyclonal antibody to TRAPPC5
アプリケーション: WB IHC
Cited expt.: IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Sheep, Dog, Chicken, Xenopus
分子量: 21 kDa(Observed); 21kD(Calculated).
ユニプロット: Q8IUR0
RRID: AB_2843159

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#DF9965-BP

TRAPPC5 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
TRAPPC5 Antibody detects endogenous levels of total TRAPPC5.
RRID:
AB_2843159
引用形式: Affinity Biosciences Cat# DF9965, RRID:AB_2843159.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

免疫原

免疫原:

A synthesized peptide derived from human TRAPPC5, corresponding to a region within the internal amino acids.

Uniprot:

Q8IUR0(TPPC5_HUMAN) >>Visit HPA database.

遺伝子(ID):
TRAPPC5(126003)
タンパク質配列:
MEARFTRGKSALLERALARPRTEVSLSAFALLFSELVQHCQSRVFSVAELQSRLAALGRQVGARVLDALVAREKGARRETKVLGALLFVKGAVWKALFGKEADKLEQANDDARTFYIIEREPLINTYISVPKENSTLNCASFTAGIVEAVLTHSGFPAKVTAHWHKGTTLMIKFEEAVIARDRALEGR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Horse
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

May play a role in vesicular transport from endoplasmic reticulum to Golgi.

細胞の位置付け:

Golgi apparatus>cis-Golgi network. Endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the TRAPP small subunits family. BET3 subfamily.

参考文献

1). MCT4 Promotes Hepatocellular Carcinoma Progression by Upregulating TRAPPC5 Gene. Journal of hepatocellular carcinoma, 2022 (PubMed: 35425722) [IF=4.1]

Application: IHC    Species: human    Sample: HCC tissue

Figure 2 TRAPPC5 is overexpressed in HCC tissue microarrays and indicated poor prognosis. (A, B) TRAPPC5 is overexpressed in HCC tissue microarrays (n=21). TRAPPC5 protein was detected in cancer tissues in higher levels, whereas paracancerous tissues expressed much lower. Scale bars, 100 μm (magnification, 100×). (C) TRAPPC5 is highly expressed in HCC tissues (n=369) compared with that in normal tissues (n=160) using the GEPIA database. (D) Kaplan-Meier analysis of overall survival showed that patients with overexpression of TRAPPC5 have a poor prognosis (n=182) using the GEPIA database. *P < 0.05.

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