ZNF280B Antibody - #DF9950

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製品: ZNF280B Antibody
カタログ: DF9950
タンパク質の説明: Rabbit polyclonal antibody to ZNF280B
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 62 kDa(Observed); 62kD(Calculated).
ユニプロット: Q86YH2
RRID: AB_2843144

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#DF9950-BP

ZNF280B Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
予測:
Pig(100%), Bovine(%), Sheep(%), Rabbit(%), Dog(%)
クローナリティ:
Polyclonal
特異性:
ZNF280B Antibody detects endogenous levels of total ZNF280B.
RRID:
AB_2843144
引用形式: Affinity Biosciences Cat# DF9950, RRID:AB_2843144.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

5''OY11.1; D87009.C22.3; SUHW2; Suppressor of hairy wing homolog 2; Z280B_HUMAN; Zinc finger protein 279; Zinc finger protein 280B; Zinc finger protein 632; ZNF279; ZNF280B; ZNF632;

免疫原

免疫原:

A synthesized peptide derived from human ZNF280B, corresponding to a region within C-terminal amino acids.

Uniprot:

Q86YH2(Z280B_HUMAN) >>Visit HPA database.

遺伝子(ID):
ZNF280B(140883)
タンパク質配列:
MEQSCEEEKEPEPQKNIQETKQVDDEDAELIFVGVEHVNEDAELIFVGVTSNSKPVVSNILNRVTPGSWSRRKKYDHLRKDTARKLQPKSHETVTSEAVTVLPASQLESRSTDSPIIIEPLSKPDYRNSSPQVVPNNSSELPSPLITFTDSLHHPVSTALSVGGINESPRVSKQLSTFEVNSINPKRAKLRDGIIEGNSSASFPSDTFHTMNTQQSTPSNNVHTSLSHVQNGAPFPAAFPKDNIHFKPINTNLDRENELAKTDILSLTSQNKTFDPKKENPIVLLSDFYYGQHKGEGQPEQKTHTTFKCLSCVKVLKNVKFMNHVKHHLEFEKQRNDSWENHTTCQHCHRQFPTPFQLQCHIENVHTAQEPSTVCKICELSFETDQVLLQHMKDHHKPGEMPYVCQVCHYRSSVFADVETHFRTCHENTKNLLCPFCLKIFKTATPYMCHYRGHWGKSAHQCSKCRLQFLTFKEKMEHKTQCHQMFKKPKQLEGLPPETKVTIQVSLEPLQPGSVDVASITVSTSDSEPSLPRSKSKISKKSH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Rabbit
100
Bovine
90
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

May function as a transcription factor.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

参考文献

1). microRNA‑196a‑3p inhibits cell proliferation and promotes cell apoptosis by targeting ADP ribosylation factor 4 in diffuse large B‑cell lymphoma. ONCOLOGY REPORTS, 2021 (PubMed: 33416178) [IF=3.8]

Application: WB    Species: Human    Sample: DLBCL cells

Figure 4. - Prediction and identification of miR-196a-3p target genes in DLBCL cells. (A) miRNA targets were predicted using TargetScan, microRNA, miRDB and DIANA-microT. (B) The protein levels of 4 miR-196a-3p candidate target genes were analyzed by western blot analysis. Up, protein bands; down, grey value. (C) mRNA levels of 4 miR-196a-3p candidate target genes were analyzed by RT-qPCR. (D) 293T cells were transiently transfected with Renilla luciferase reporter vectors containing either wild-type or mutant ARF4 3′-UTR seed sequence, and luciferase activity was measured following co-transfection with miR-196a-3p or NC. *P

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