Phospho-SRF (Ser99)[Ser103] Antibody - #AF3160
![Phospho-SRF (Ser99)[Ser103] Antibody - AF3160 at 1/200 staining human testis tissue sections by IHC-P.](http://img.affbiotech.cn/images/201711/thumb_img/1341_thumb_P_1511514219774.jpg "AF3160 at 1/200 staining human testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.")
![Phospho-SRF (Ser99)[Ser103] Antibody - AF3160 staining Hela cells(Etoposide 25μm 5h) by IF/ICC.](http://img.affbiotech.cn/images/202205/af3160_57572_phospho_srf_ser99_ser103_antibody_1653270086.jpg "AF3160 staining Hela cells(Etoposide 25μm 5h) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(#AF3160) and mouse anti-beta tubulin Ab(#T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody.
The nuclear counter stain is DAPI (blue).")
![Phospho-SRF (Ser99)[Ser103] Antibody - peptide-ELISA analysis of AF3160.](http://img.affbiotech.cn/images/pelisa/809/af3160-peptide-elisa.png "peptide-ELISA analysis of AF3160. showing specificity to antigen peptide. Peptides concentration: 1ug/ml.<br>
P-peptide: phospho-peptide. N-peptide: non-phospho-peptide.")
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3160, RRID:AB_2834595.
折りたたみ／展開
c fos serum response element binding factor; c fos serum response element binding transcription factor; ELK3; ERP; MCM 1; MCM1; OTTHUMP00000039820; SAP2; Serum response factor; SRF; SRF serum response factor c fos serum response element binding transcription factor; SRF_HUMAN;
免疫原
A synthesized peptide derived from human SRF around the phosphorylation site of Ser99.
- P11831 SRF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLPTQAGAAAALGRGSALGGSLNRTPTGRPGGGGGTRGANGGRVPGNGAGLGPGRLEREAAAAAATTPAPTAGALYSGSEGDSESGEEEELGAERRGLKRSLSEMEIGMVVGGPEASAAATGGYGPVSGAVSGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRSDPTTDQRMSATGFEETDLTYQVSESDSSGETKDTLKPAFTVTNLPGTTSTIQTAPSTSTTMQVSSGPSFPITNYLAPVSASVSPSAVSSANGTVLKSTGSGPVSSGGLMQLPTSFTLMPGGAVAQQVPVQAIQVHQAPQQASPSRDSSTDLTQTSSSGTVTLPATIMTSSVPTTVGGHMMYPSPHAVMYAPTSGLGDGSLTVLNAFSQAPSTMQVSHSQVQEPGGVPQVFLTASSGTVQIPVSAVQLHQMAVIGQQAGSSSNLTELQVVNLDTAHSTKSE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
研究背景
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.
Phosphorylated by PRKDC.
Nucleus.
研究領域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
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