SLC6A20 Antibody - #DF9926

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製品: SLC6A20 Antibody
カタログ: DF9926
タンパク質の説明: Rabbit polyclonal antibody to SLC6A20
アプリケーション: WB IHC
Cited expt.:
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 66 kDa(Observed); 66kD(Calculated).
ユニプロット: Q9NP91
RRID: AB_2843120

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#DF9926-BP

SLC6A20 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
予測:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
クローナリティ:
Polyclonal
特異性:
SLC6A20 Antibody detects endogenous levels of total SLC6A20.
RRID:
AB_2843120
引用形式: Affinity Biosciences Cat# DF9926, RRID:AB_2843120.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

免疫原

免疫原:

A synthesized peptide derived from human SLC6A20, corresponding to a region within the internal amino acids.

Uniprot:

Q9NP91(S6A20_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC6A20(54716)
発現特異性:
Q9NP91 S6A20_HUMAN:

Kidney and small intestine. Expressed in the S3 segment of the proximal tubule.

タンパク質配列:
MEKARPLWANSLQFVFACISYAVGLGNVWRFPYLCQMYGGGSFLVPYIIMLIVEGMPLLYLELAVGQRMRQGSIGAWRTISPYLSGVGVASVVVSFFLSMYYNVINAWAFWYLFHSFQDPLPWSVCPLNGNHTGYDEECEKASSTQYFWYRKTLNISPSLQENGGVQWEPALCLLLAWLVVYLCILRGTESTGKVVYFTASLPYCVLIIYLIRGLTLHGATNGLMYMFTPKIEQLANPKAWINAATQIFFSLGLGFGSLIAFASYNEPSNNCQKHAIIVSLINSFTSIFASIVTFSIYGFKATFNYENCLKKVSLLLTNTFDLEDGFLTASNLEQVKGYLASAYPSKYSEMFPQIKNCSLESELDTAVQGTGLAFIVYTEAIKNMEVSQLWSVLYFFMLLMLGIGSMLGNTAAILTPLTDSKIISSHLPKEAISGLVCLVNCAIGMVFTMEAGNYWFDIFNDYAATLSLLLIVLVETIAVCYVYGLRRFESDLKAMTGRAVSWYWKVMWAGVSPLLIVSLFVFYLSDYILTGTLKYQAWDASQGQLVTKDYPAYALAVIGLLVASSTMCIPLAALGTFVQRRLKRGDADPVA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
89
Xenopus
67
Chicken
67
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Mediates the calcium-dependent uptake of imino acids such as L-proline, N-methyl-L-proline and pipecolate as well as N-methylated amino acids. Involved in the transport of glycine.

細胞の位置付け:

Apical cell membrane>Multi-pass membrane protein.
Note: Located in the apical brush border membrane of kidney proximal tubule cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Kidney and small intestine. Expressed in the S3 segment of the proximal tubule.

タンパク質ファミリー:

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A20 subfamily.

参考文献

1). Serine and glycine physiology reversibly modulate retinal and peripheral nerve function. Cell metabolism, 2024 (PubMed: 39191258) [IF=27.7]

Application: IF/ICC    Species: Mouse    Sample:

Figure 2 Retinal serine is predominately obtained from the circulation (A) % serine M3 label in tissue normalized to % serine (ser) M3 label in plasma after mice were infused with [U-13C3]serine for 2 h (n = 4). (B) Abundance of serine in plasma and tissue relative to levels when serine is infused at 2 nmol/min/g mice (n = 5 for 2 nmol/min/g and n = 4 for 10 nmol/min/g groups). (C) % glycine M2 label in tissue normalized to % glycine (gly) M2 label in plasma after mice were infused with [U-13C2]glycine for 2 h (n = 4). (D) Gene expression of Slc1a4, Slc6a20a, and Slc6a20b per copy of housekeeping gene (36b4) across tissue types (n = 5). (E) Immunofluorescence visualization of SLC1A4 in the mouse retina (SLC1A4, magenta; glutamine synthetase [GS], green; DAPI, blue). ONL, outer nuclear layer; INL, inner nuclear layer; RPE, retinal pigment epithelium. White arrows indicate co-staining of Muller glia. Schematic prepared in BioRender. (F) Immunofluorescence visualization of SLC6A20 in mouse retina (SLC6A20, red; glutamine synthetase [GS], green; DAPI, blue). ONL, outer nuclear layer; INL, inner nuclear layer; RPE, retinal pigment epithelium. Data are mean ± SD (A–C) or mean ± SEM (D) and were analyzed using one-way ANOVA. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

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