NOL7 Antibody - #DF9720

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製品: NOL7 Antibody
カタログ: DF9720
タンパク質の説明: Rabbit polyclonal antibody to NOL7
アプリケーション: WB IHC
Cited expt.: IHC
反応性: Human, Mouse, Rat
予測: Pig, Horse, Rabbit, Dog
分子量: 29 kDa; 29kD(Calculated).
ユニプロット: Q9UMY1
RRID: AB_2842915

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Horse(91%), Rabbit(91%), Dog(91%)
クローナリティ:
Polyclonal
特異性:
NOL7 Antibody detects endogenous levels of total NOL7.
RRID:
AB_2842915
引用形式: Affinity Biosciences Cat# DF9720, RRID:AB_2842915.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C6orf90; dJ223E5.2; Nol7; NOL7_HUMAN; NOP27; Nucleolar protein 7; Nucleolar protein of 27 kDa; PQBP3; RARG-1;

免疫原

免疫原:

A synthesized peptide derived from human NOL7, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9UMY1(NOL7_HUMAN) >>Visit HPA database.

遺伝子(ID):
NOL7(51406)
発現特異性:
Q9UMY1 NOL7_HUMAN:

Expressed in numerous tissues. Particularly prevalent in the adrenal gland, thyroid gland, heart and skeletal muscle.

タンパク質配列:
MVQLRPRASRAPASAEAMVDEGQLASEEEEAEHGLLLGQPSSGAAAEPLEEDEEGDDEFDDEAPEELTFASAQAEAREEERRVRETVRRDKTLLKEKRKRREELFIEQKKRKLLPDTILEKLTTASQTNIKKSPGKVKEVNLQKKNEDCEKGNDSKKVKVQKVQSVSQNKSYLAVRLKDQDLRDSRQQAAQAFIHNSLYGPGTNRTTVNKFLSLANKRLPVKRAAVQFLNNAWGIQKKQNAKRFKRRWMVRKMKTKK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
91
Dog
91
Rabbit
91
Bovine
73
Sheep
73
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

細胞の位置付け:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in numerous tissues. Particularly prevalent in the adrenal gland, thyroid gland, heart and skeletal muscle.

参考文献

1). NOL-7 serves as a potential prognostic-related biomarker for hepatocellular carcinoma. Discover oncology, 2025 (PubMed: 39836310) [IF=2.2]

Application: IHC    Species: human    Sample:

Fig. 2 NOL7 expression from our validation set. A qRT-PCR, using t-tests of unpaired samples. B qRT-PCR, using t-test paired samples. C HE images of HCC tumor tissue and adjacent noncancerous tissue (left). D IHC images and expression of NOL7 protein in HCC tumor tissues and adjacent noncancerous tissues (right), Magnification =  × 400, The positive stain appears brown. E Using t-tests of unpaired samples. F Using a paired t-test sample. *P 

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