GPM6A Antibody - #DF9699

Fig. 7. Knockdown of PLCβ4 rescues cognitive and memory deficits and neuronal loss induced by maternal Cd exposure. A) The schedule of knocking down PLCβ4 in the hippocampus of male offspring rats under Cd exposure. B-E) Y-maze test is used to assess the short-term learning and memory abilities of rats. Representative movement tracks C), spontaneous alternation B) total distance D), and number of entries into each arm E) are recorded, n = 6/9. F) The representative movement tracks of escape latency on day 5 in navigation test of MWM. G) The escape latencies of rats. H-J) The representative movement tracks in spatial probe test (H), times of crossing removed-platform area I), and time spent in the target quadrant J), n = 6/8. K-L) Anti-NeuN (green) staining exhibited the survive neurons in the DG regions of male rats exposed to maternal Cd, and the positive cell density were analyzed, n = 3/4. M) Golgi staining was used to measure the neuronal structure in the brain of male offspring, Scale: 20 µm. N) Representative the statistics analysis of average neurite length, neurite number of Golgi-stained neurons, n = 27–41 neurons per group from 3 to 4 rats. O) The expressions of PLCβ4, p-PLCγ1, p-CREB, BDNF, p-TrkB, TrkB, GPM6A and β-actin in the hippocampus are detected by western blotting. P) Relative quantification of PLCβ4, p-PLCγ1, p-CREB, BDNF, p-TrkB, TrkB, GPM6A, n = 6/9. Data are represented as mean ± SEM. Statistical significance are calculated by one-way ANOVA with Tukey’s post-hoc test. * P