MYH3 Antibody - #DF9647

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製品: MYH3 Antibody
カタログ: DF9647
タンパク質の説明: Rabbit polyclonal antibody to MYH3
アプリケーション: WB IHC IF/ICC
Cited expt.: IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit
分子量: 224kDa; 224kD(Calculated).
ユニプロット: P11055
RRID: AB_2842843

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%)
クローナリティ:
Polyclonal
特異性:
MYH3 Antibody detects endogenous levels of total MYH3.
RRID:
AB_2842843
引用形式: Affinity Biosciences Cat# DF9647, RRID:AB_2842843.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

adult 1; MYH1; MYH1_HUMAN; MYH2; MYH3; MYH4; MYH6; MyHC-2x; MyHC-IIx/d; MYL1; MYL2; MYL3; MYL5; MYL6; Myosin light chain 2 regulatory cardiac slow; Myosin 1; Myosin heavy chain 1; Myosin heavy chain 1 skeletal muscle adult; Myosin heavy chain 2 skeletal muscle adult; Myosin heavy chain 2x; Myosin heavy chain 3 skeletal muscle adult; Myosin heavy chain 4 skeletal muscle adult; Myosin heavy chain 6 skeletal muscle adult; Myosin heavy chain; Myosin heavy chain IIx/d; Myosin heavy chain skeletal muscle adult 1; Myosin II; Myosin light chain 1 alkali skeletal fast; Myosin light chain 3 alkali ventricular skeletal slow; Myosin light chain 4 alkali atrial embryonic; Myosin light chain 5 regulatory; Myosin light chain 6 alkali smooth muscle and non muscle; Myosin-1; Myosin1; skeletal muscle;

免疫原

免疫原:

A synthesized peptide derived from human MYH3, corresponding to a region within C-terminal amino acids.

Uniprot:

P11055(MYH3_HUMAN) >>Visit HPA database.

遺伝子(ID):
MYH3(4621)
発現特異性:
P11055 MYH3_HUMAN:

Expressed in fetal bone, thymus, placenta, heart, brain, and liver.

タンパク質配列:
MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYAKGKIKSSQDGKVTVETEDNRTLVVKPEDVYAMNPPKFDRIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVEGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKDSKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGLYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKVVKGRAEAHFSLIHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATADADSGKKKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDDRLAKLITRTQAVCRGFLMRVEFQKMVQRRESIFCIQYNIRSFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEELSGLDETIAKLTREKKALQEAHQQALDDLQAEEDKVNSLNKTKSKLEQQVEDLESSLEQEKKLRVDLERNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDFEYCQLQSKVEDEQTLGLQFQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEAMVAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSMESVSKSKANLEKICRTLEDQLSEARGKNEEIQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQTEELKRQLEEENKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEASLKESRSLSTELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKTIHELEKSRKQIELEKADIQLALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRNEAIRLKKKMEGDLNEIEIQLSHANRQAAETLKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRANLLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLMQLQSEVEDASRDARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRIRELEFELEGEQKKNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEADEQANAHLTKFRKAQHELEEAEERADIAESQVNKLRAKTRDFTSSRMVVHESEE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
91
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Muscle contraction.

細胞の位置付け:

Cytoplasm>Myofibril.
Note: Thick filaments of the myofibrils.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in fetal bone, thymus, placenta, heart, brain, and liver.

タンパク質ファミリー:

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

参考文献

1). miR-103-3p Regulates the Differentiation and Autophagy of Myoblasts by Targeting MAP4. International journal of molecular sciences, 2023 (PubMed: 36835542) [IF=5.6]

Application: IF/ICC    Species: Mouse    Sample: C2C12 cells

Figure 2. miR-103-3p inhibited the differentiation of C2C12 cells. (A,C) The mRNA expression of MyoG, MyoD, and MyHC in C2C12 cells was determined by qRT-PCR at 24 h after transfection with the miR-103-3p inhibitor or mimic. n = 3. (B,D) The protein expression of MyoG in C2C12 cells was determined by western blot at 48 h after transfection with the miR-103-3p inhibitor or mimic. β-Actin was used as the endogenous control. n = 3 (E,F) C2C12 cells were induced to differentiate for 48 h. Anti-MyHC immunofluorescence staining was performed in C2C12 cells at 48 h after transfection with the miR-103-3p mimic or inhibitor, and the relative myotube area was measured following miR-103-3p knockdown and overexpression. Scale bar = 100 μm. n = 3. * p < 0.05, ** p < 0.01.

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