(1)
Control Products
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
クローナリティ:
Polyclonal
特異性:
USP54 Antibody detects endogenous levels of total USP54.
RRID:
AB_2842791
引用形式: Affinity Biosciences Cat# DF9595, RRID:AB_2842791.
引用形式: Affinity Biosciences Cat# DF9595, RRID:AB_2842791.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
USP54; bA137L10.3; bA137L10.4; C10orf29; FLJ37318; Inactive ubiquitin carboxyl-terminal hydrolase 54; nactive ubiquitin-specific peptidase 54;
免疫原
免疫原:
A synthesized peptide derived from human USP54, corresponding to a region within the internal amino acids.
Uniprot:
遺伝子(ID):
発現特異性:
タンパク質配列:
- Q70EL1 UBP54_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Horse
91
Xenopus
91
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
研究背景
機能:
Has no peptidase activity.
組織特異性:
Weakly expressed in a few tissues.
タンパク質ファミリー:
Belongs to the peptidase C19 family.
参考文献
1). Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4. iScience, 2021
(PubMed: 36590171)
[IF=5.8]
Application: WB Species: Human Sample: SGC7901 cells
Figure 5 USP54 combines with PLK4 and mediates its deubiquitination (A) Microarray analysis of mRNAs extracted from SGC7901-vector and SGC7901-CEP120 cells. (B) Results of the ubiquitination level of PLK4 in HEK293T cells, which were co-transfected with Flag-PLK4, HA-Ub, and one of the three DUBs. (C) WB results of USP54 and PLK4 expression in GC tissues. p value derived from Pearson correlation analysis. (D) WB results of PLK4 and USP54 after USP54 overexpression or knockdown. (E) CoIP analysis of the interaction between PLK4 and USP54 in SGC7901 and BGC823 cells. (F) HEK 293T cells were co-transfected with His-USP54 and Flag-PLK4 full length or Flag-PLK4 (1-740), Flag-PLK4 (1-270), and Flag-PLK4 (270-890) constructs and harvested for WB and IP analysis. (G) HEK 293T cells were co-transfected with His-USP54 and Flag-PLK4 wildtype or Flag-PLK4 lysine residue mutants and harvested for IP analysis. (H) Ubiquitination level of PLK4 after USP54 overexpression or knockdown examined by ubiquitination assay. (I) WB results of PLK4 in SGC7901 cells transfected with Lenti-CEP120 and siUSP54 and BGC823 cells transfected with Lenti-shCEP120 and USP54 plasmid.
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