製品: GJB5 Antibody
カタログ: DF9528
タンパク質の説明: Rabbit polyclonal antibody to GJB5
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Horse
分子量: 31 kDa; 31kD(Calculated).
ユニプロット: O95377
RRID: AB_2842724

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製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(88%), Horse(86%)
クローナリティ:
Polyclonal
特異性:
GJB5 Antibody detects endogenous levels of total GJB5.
RRID:
AB_2842724
引用形式: Affinity Biosciences Cat# DF9528, RRID:AB_2842724.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Connexin 31.1; Connexin-31.1; Connexin31.1; Cx31.1; CXB5_HUMAN; Gap junction beta 5 protein; Gap junction beta-5 protein; Gap junction protein beta 5 31.1kDa; Gap junction protein beta 5; Gap junctional protein cx31.1; GJB 5; GJB5;

免疫原

免疫原:

A synthesized peptide derived from human GJB5, corresponding to a region within C-terminal amino acids.

Uniprot:
遺伝子(ID):
タンパク質配列:
MNWSIFEGLLSGVNKYSTAFGRIWLSLVFIFRVLVYLVTAERVWSDDHKDFDCNTRQPGCSNVCFDEFFPVSHVRLWALQLILVTCPSLLVVMHVAYREVQEKRHREAHGENSGRLYLNPGKKRGGLWWTYVCSLVFKASVDIAFLYVFHSFYPKYILPPVVKCHADPCPNIVDCFISKPSEKNIFTLFMVATAAICILLNLVELIYLVSKRCHECLAARKAQAMCTGHHPHGTTSSCKQDDLLSGDLIFLGSDSHPPLLPDRPRDHVKKTIL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
86
Bovine
75
Sheep
75
Dog
75
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the connexin family. Beta-type (group I) subfamily.

参考文献

1). Alteration of LncRNA expression in mice placentae after frozen embryo transfer is associated with increased fetal weight. Reproductive Biology, 2022 (PubMed: 35567965) [IF=2.5]

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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