製品: FOLR2 Antibody
カタログ: DF9518
タンパク質の説明: Rabbit polyclonal antibody to FOLR2
アプリケーション: WB IF/ICC
反応性: Human, Mouse
予測: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 29 kDa; 29kD(Calculated).
ユニプロット: P14207
RRID: AB_2842714

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(88%)
クローナリティ:
Polyclonal
特異性:
FOLR2 Antibody detects endogenous levels of total FOLR2.
RRID:
AB_2842714
引用形式: Affinity Biosciences Cat# DF9518, RRID:AB_2842714.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

beta hFR; FBP; FBP/PL 1; FBP2; fetal/placental; folate receptor 2 (fetal); Folate receptor 2; Folate receptor; Folate receptor beta; Folate receptor, fetal/placental; Folbp 2; Folbp2; Folr2; FOLR2_HUMAN; FR beta; FR P3; FR-beta; Placental folate binding protein; Placental folate-binding protein;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P14207 FOLR2_HUMAN:

Expressed in placenta and hematopoietic cells. Expression is increased in malignant tissues.

タンパク質配列:
MVWKWMPLLLLLVCVATMCSAQDRTDLLNVCMDAKHHKTKPGPEDKLHDQCSPWKKNACCTASTSQELHKDTSRLYNFNWDHCGKMEPACKRHFIQDTCLYECSPNLGPWIQQVNQSWRKERFLDVPLCKEDCQRWWEDCHTSHTCKSNWHRGWDWTSGVNKCPAGALCRTFESYFPTPAALCEGLWSHSYKVSNYSRGSGRCIQMWFDSAQGNPNEEVARFYAAAMHVNAGEMLHGTGGLLLSLALMLQLWLLG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
88
Bovine
88
Sheep
88
Dog
88
Pig
63
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14207 基板として

Site PTM Type Enzyme
N115 N-Glycosylation
N195 N-Glycosylation

研究背景

機能:

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release.

PTMs:

N-glycosylated.

細胞の位置付け:

Cell membrane>Lipid-anchor. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in placenta and hematopoietic cells. Expression is increased in malignant tissues.

タンパク質ファミリー:

Belongs to the folate receptor family.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

参考文献

1). Metabolic reprogramming of proinflammatory macrophages by target delivered roburic acid effectively ameliorates rheumatoid arthritis symptoms. Signal Transduction and Targeted Therapy, 2023 (PubMed: 37500654) [IF=39.3]

Application: IF/ICC    Species: Mouse    Sample: RAW264.7 cells

Fig. 2 RBA-NPs display targeting capability in vitro. a, b Confocal micrographs showed that DiD-NPs (red) enjoyed significantly improved cell uptake compared with free DiD in LPS + IFN-γ activated RAW264.7 cells. Scale bar = 10 μm. c Flow cytometry analysis showed that the fluorescence intensity of DiD-NPs in LPS + IFN-γ activated RAW264.7 cells was higher than that of free DiD in RAW264.7. d The expression of CD44 and folate receptors increased significantly on the surface of LPS + IFN-γ activated RAW264.7 cells (d). Scale bar = 10 μm. e LPS + IFN-γ activated macrophages were pretreated with HA or FA or both HA and FA to compete for the overexpressed CD44 or folate receptors, and cellular uptake of DiD-NPs was reduced. f Confocal micrographs showed co-localization of DiD-NPs (orange) with CD44 receptor (red) and folate receptor (green). Scale bar = 20 μm. Cell nuclei was stained with DAPI (blue). All results are shown as mean ± SD. *P 

2). Reactive oxygen species-responsive dexamethasone-loaded nanoparticles for targeted treatment of rheumatoid arthritis via suppressing the iRhom2/TNF-α/BAFF signaling pathway. Biomaterials, 2020 (PubMed: 31918224) [IF=14.0]

Application: WB    Species: Mouse    Sample: Raw264.7 and MH7A cells

Fig. 3. In vitro cellular uptake of Cy5-labeled NPs. (A) Raw264.7 macrophages induced with LPS (1 μg/mL) for 6 h. Then cellular uptake of Cy5-labeled Dex/Oxi- αCD NPs or Dex/FA-Oxi-αCD NPs was assessed using confocal laser scanning microscopy at various times. Nuclei were counterstained with DAPI (blue). Scale bars, 20 μm. (B) Quantitative analysis of the corresponding fluorescence intensity of intracellular NPs (red) in Raw264.7 cells. (C) The mRNA expression of FRα, FRβ, and FRγ in MH7A cells were analyzed by quantitative real-time PCR. Data were presented by the relative amount of mRNA normalized by GAPDH. (D) Western blot analysis of FRα, FRβ and FRγ expression in Raw264.7 and MH7A cells. α-Tubulin was used as a loading control. (E–F) Cellular uptake (E) and corresponding fluorescence intensity (F) of Cy5-labeled Dex/Oxi-NPs or Dex/FA-Oxi-NPs in MH7A cells at various time points. *p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

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