| 製品: | NSMCE2 Antibody |
| カタログ: | DF9475 |
| タンパク質の説明: | Rabbit polyclonal antibody to NSMCE2 |
| アプリケーション: | WB IHC |
| 反応性: | Human |
| 予測: | Rabbit |
| 分子量: | 28 kDa(Observed); 28kD(Calculated). |
| ユニプロット: | Q96MF7 |
| RRID: | AB_2842671 |
Control Products
製品説明
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9475, RRID:AB_2842671.
折りたたみ／展開
C8orf36; E3 SUMO-protein ligase NSE2; hMMS21; hNSE2; methyl methanesulfonate sensitivity gene 21; MMS21; MMS21 homolog; non-SMC element 2; Non-SMC element 2 homolog; Non-structural maintenance of chromosomes element 2 homolog; NSE2_HUMAN; Nsmce2;
免疫原
A synthesized peptide derived from human NSMCE2, corresponding to a region within C-terminal amino acids.
- Q96MF7 NSE2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGRSSSNSGSTGFISFSGVESALSSLKNFQACINSGMDTASSVALDLVESQTEVSSEYSMDKAMVEFATLDRQLNHYVKAVQSTINHVKEERPEKIPDLKLLVEKKFLALQSKNSDADFQNNEKFVQFKQQLKELKKQCGLQADREADGTEGVDEDIIVTQSQTNFTCPITKEEMKKPVKNKVCGHTYEEDAIVRMIESRQKRKKKAYCPQIGCSHTDIRKSDLIQDEALRRAIENHNKKRHRHSE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
研究背景
E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as an E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, and maybe the cohesin components RAD21 and STAG2. Required for recruitment of telomeres to PML nuclear bodies. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair, and for formation of APBs in ALT cell lines. Required for sister chromatid cohesion during prometaphase and mitotic progression.
Sumoylated, possibly via autosumoylation.
Nucleus. Chromosome>Telomere. Nucleus>PML body.
Note: Localizes to PML nuclear bodies in ALT cell lines.
Belongs to the NSE2 family.
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