製品: RPC7 Antibody
カタログ: DF9461
タンパク質の説明: Rabbit polyclonal antibody to RPC7
アプリケーション: WB IHC IF/ICC
反応性: Human
予測: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 26 kDa; 26kD(Calculated).
ユニプロット: O15318
RRID: AB_2842657

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(86%), Xenopus(83%)
クローナリティ:
Polyclonal
特異性:
RPC7 Antibody detects endogenous levels of total RPC7.
RRID:
AB_2842657
引用形式: Affinity Biosciences Cat# DF9461, RRID:AB_2842657.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

DNA directed RNA polymerase III 32 kDa polypeptide; DNA-directed RNA polymerase III subunit G; DNA-directed RNA polymerase III subunit RPC7; Polr3g; Polymerase (RNA) III (DNA directed) polypeptide G (32kD); RNA polymerase III 32 kDa subunit; RNA polymerase III subunit C7; RPC32; RPC7; RPC7_HUMAN;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
O15318 RPC7_HUMAN:

Barely detectable in differentiated tissues. Expressed in embryonic stem cells and in other dividing cells, such as some tumor cell lines.

タンパク質配列:
MAGNKGRGRAAYTFNIEAVGFSKGEKLPDVVLKPPPLFPDTDYKPVPLKTGEGEEYMLALKQELRETMKRMPYFIETPEERQDIERYSKRYMKVYKEEWIPDWRRLPREMMPRNKCKKAGPKPKKAKDAGKGTPLTNTEDVLKKMEELEKRGDGEKSDEENEEKEGSKEKSKEGDDDDDDDAAEQEEYDEEEQEEENDYINSYFEDGDDFGADSDDNMDEATY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Chicken
86
Xenopus
83
Zebrafish
67
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15318 基板として

Site PTM Type Enzyme
R7 Methylation
R9 Methylation
K33 Ubiquitination
K44 Ubiquitination
K61 Ubiquitination
T67 Phosphorylation
Y73 Phosphorylation
K89 Acetylation
K118 Acetylation
K122 Acetylation
T133 Phosphorylation
T136 Phosphorylation
S157 Phosphorylation

研究背景

機能:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs), induce type I interferon and NF- Kappa-B through the RIG-I pathway.

細胞の位置付け:

Nucleus. Cytoplasm.
Note: Excluded from nucleoli (PubMed:21898682). In zygotes and the 2-cell stage embryos, mainly in the cytoplasm. Starts to localize to the nucleus in the 8-16 cell stage embryo and early blastocysts (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Barely detectable in differentiated tissues. Expressed in embryonic stem cells and in other dividing cells, such as some tumor cell lines.

サブユニット構造:

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex. Directly interacts with POLR3C/RPC62. Also found a trimeric complex with POLR3C and POLR3GL.

タンパク質ファミリー:

Belongs to the eukaryotic RPC7 RNA polymerase subunit family.

研究領域

· Genetic Information Processing > Transcription > RNA polymerase.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

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