製品: DYNC1I2 Antibody
カタログ: DF9427
タンパク質の説明: Rabbit polyclonal antibody to DYNC1I2
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Horse, Sheep, Dog, Xenopus
分子量: 71 kDa; 71kD(Calculated).
ユニプロット: Q13409
RRID: AB_2842623

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
DYNC1I2 Antibody detects endogenous levels of total DYNC1I2.
RRID:
AB_2842623
引用形式: Affinity Biosciences Cat# DF9427, RRID:AB_2842623.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Cytoplasmic dynein 1 intermediate chain 2; Cytoplasmic dynein intermediate chain 2; cytosolic; DC1I2_HUMAN; DH IC 2; DH IC-2; DNCI2; DNCIC2; Dync1i2; Dynein intermediate chain 2; Dynein intermediate chain 2, cytosolic; Dynein, cytoplasmic, intermediate polypeptide 2; IC2;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAVAPVQEESDLEKKRREAEALLQSMGLTPESPIVFSEYWVPPPMSPSSKSVSTPSEAGSQDSGDGAVGSRTLHWDTDPSVLQLHSDSDLGRGPIKLGMAKITQVDFPPREIVTYTKETQTPVMAQPKEDEEEDDDVVAPKPPIEPEEEKTLKKDEENDSKAPPHELTEEEKQQILHSEEFLSFFDHSTRIVERALSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHRVVSCLDWSSQYPELLVASYNNNEDAPHEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAKFHPNLVVGGTYSGQIVLWDNRSNKRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQDSMELVHKQSKAVAVTSMSFPVGDVNNFVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHLFVTSSFDWTVKLWTTKNNKPLYSFEDNADYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASISVEGNPALNRVRWTHSGREIAVGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINANRADAEEEAATRIPA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
100
Bovine
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13409 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K8 Acetylation
K8 Ubiquitination
S51 Phosphorylation
S73 Phosphorylation
S87 Phosphorylation P28482 (MAPK1)
S89 Phosphorylation
S90 Phosphorylation
S92 Phosphorylation
S94 Phosphorylation
T95 Phosphorylation
S97 Phosphorylation
S101 Phosphorylation
S104 Phosphorylation
K137 Ubiquitination
T162 Phosphorylation
K182 Ubiquitination
K191 Ubiquitination
T209 Phosphorylation
S219 Phosphorylation
S238 Phosphorylation
K255 Ubiquitination
K264 Ubiquitination
R269 Methylation
K427 Ubiquitination

研究背景

機能:

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes. Has a critical role in neurodevelopment.

PTMs:

The phosphorylation status of Ser-90 appears to be involved in dynactin-dependent target binding.

細胞の位置付け:

Cytoplasm>Cytoskeleton. Cytoplasm.
Note: Detected in the cytoplasm of pachytene spermatocytes. Localizes to the manchette in elongating spermatids.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homodimer (By similarity). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNLT1 and DYNLT3. Interacts with DCNT1 (By similarity). Interacts with BICD2 (By similarity). Interacts with SPEF2 (By similarity).

(Microbial infection) Interacts with human adenovirus 5 hexon protein; this interaction probably allows virus intracellular transport.

タンパク質ファミリー:

Belongs to the dynein intermediate chain family.

研究領域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

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