製品: CTH Antibody
カタログ: DF9421
タンパク質の説明: Rabbit polyclonal antibody to CTH
アプリケーション: WB IHC
反応性: Human, Mouse
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 45 kDa; 45kD(Calculated).
ユニプロット: P32929
RRID: AB_2842617

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(90%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
CTH Antibody detects endogenous levels of total CTH.
RRID:
AB_2842617
引用形式: Affinity Biosciences Cat# DF9421, RRID:AB_2842617.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

CGL_HUMAN; CTH; cystathionase (cystathionine gamma-lyase); Cystathionine gamma lyase; Cystathionine gamma-lyase; Cysteine desulfhydrase; Gamma cystathionase; Gamma-cystathionase; Homoserine deaminase; Homoserine dehydratase; MGC9471;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGHSDVVMGLVSVNCESLHNRLRFLQNSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQHELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Pig
90
Chicken
89
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P32929 基板として

Site PTM Type Enzyme
S8 Phosphorylation
K48 Ubiquitination
Y60 Phosphorylation
K73 Ubiquitination
K139 Ubiquitination
K141 Ubiquitination
K152 Ubiquitination
K212 Acetylation
K288 Ubiquitination
K304 Ubiquitination
K330 Ubiquitination
S377 Phosphorylation Q13237 (PRKG2)
K395 Ubiquitination

研究背景

機能:

Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homotetramer. Interacts with CALM in a calcium-dependent manner (By similarity).

タンパク質ファミリー:

Belongs to the trans-sulfuration enzymes family.

研究領域

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Metabolism of other amino acids > Selenocompound metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

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