CHAC1 Antibody - #DF9353

(2)   (4)  
製品: CHAC1 Antibody
カタログ: DF9353
タンパク質の説明: Rabbit polyclonal antibody to CHAC1
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 29kDa; 24kD(Calculated).
ユニプロット: Q9BUX1
RRID: AB_2842549

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(83%)
クローナリティ:
Polyclonal
特異性:
CHAC1 Antibody detects endogenous levels of total CHAC1.
RRID:
AB_2842549
引用形式: Affinity Biosciences Cat# DF9353, RRID:AB_2842549.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Blocks Notch protein; Botch; Cation transport regulator like protein 1; Cation transport regulator-like protein 1; ChaC; ChaC cation transport regulator homolog 1; ChaC cation transport regulator like 1; chac1; CHAC1_HUMAN; Gamma GCT acting on glutathione homolog 1; Gamma-GCG 1; Glutathione-specific gamma-glutamylcyclotransferase 1;

免疫原

免疫原:

A synthesized peptide derived from human CHAC1, corresponding to a region within the internal amino acids.

Uniprot:

Q9BUX1(CHAC1_HUMAN) >>Visit HPA database.

遺伝子(ID):
CHAC1(79094)
タンパク質配列:
MKQESAAPNTPPTSQSPTPSAQFPRNDGDPQALWIFGYGSLVWRPDFAYSDSRVGFVRGYSRRFWQGDTFHRGSDKMPGRVVTLLEDHEGCTWGVAYQVQGEQVSKALKYLNVREAVLGGYDTKEVTFYPQDAPDQPLKALAYVATPQNPGYLGPAPEEAIATQILACRGFSGHNLEYLLRLADFMQLCGPQAQDEHLAAIVDAVGTMLPCFCPTEQALALV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
83
Chicken
83
Zebrafish
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos.

細胞の位置付け:

Cytoplasm>Cytosol. Golgi apparatus>trans-Golgi network.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the gamma-glutamylcyclotransferase family. ChaC subfamily.

参考文献

1). Identification of Ferroptotic Genes in Spinal Cord Injury at Different Time Points: Bioinformatics and Experimental Validation. Molecular Neurobiology, 2022 (PubMed: 35796899) [IF=4.6]
2). CPEB1 enhances erastin‐induced ferroptosis in gastric cancer cells by suppressing twist1 expression. IUBMB LIFE, 2021 (PubMed: 34184391) [IF=3.7]

Application: WB    Species: Mice    Sample: GC cells

FIGURE 4 CPEB1 suppressed twist1 expression in GC cells. (a-f) After CPEB1-silenced AGS cells and CPEB1-overexpressed HGC-27 cells were treated with erastin (10 μg/ml) for 24 h, twist1, ATF4, and CHAC1 protein expression levels were measured by western blot. N = 3. Data are shown as means ± SD. **p < 0.01, and ***p < 0.001. ATF4, activating transcription factor 4; CPEB1, cytoplasmic polyadenylation element binding protein 1; CHAC1, ChaC glutathione-specific γ glutamylcyclotransferase 1; GC, gastric cancer

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.