CATSPER4 Antibody - #DF9351

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製品: CATSPER4 Antibody
カタログ: DF9351
タンパク質の説明: Rabbit polyclonal antibody to CATSPER4
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human
予測: Pig, Horse, Sheep, Rabbit
分子量: 50~60kDa; 54kD(Calculated).
ユニプロット: Q7RTX7
RRID: AB_2842547

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製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(88%), Horse(88%), Sheep(88%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
CATSPER4 Antibody detects endogenous levels of total CATSPER4.
RRID:
AB_2842547
引用形式: Affinity Biosciences Cat# DF9351, RRID:AB_2842547.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Cation channel sperm associated 4; Cation channel sperm associated protein 4; CatSper4; Channel sperm associated 4; Ion channel CatSper4; MGC149223;

免疫原

免疫原:

A synthesized peptide derived from human CATSPER4, corresponding to a region within the internal amino acids.

Uniprot:

Q7RTX7(CTSR4_HUMAN) >>Visit HPA database.

遺伝子(ID):
CATSPER4(378807)
発現特異性:
Q7RTX7 CTSR4_HUMAN:

Testis-specific.

タンパク質配列:
MRDNEKAWWQQWTSHTGLEGWGGTQEDRMGFGGAVAALRGRPSPLQSTIHESYGRPEEQVLINRQEITNKADAWDMQEFITHMYIKQLLRHPAFQLLLALLLVINAITIALRTNSYLDQKHYELFSTIDDIVLTILLCEVLLGWLNGFWIFWKDGWNILNFIIVFILLLRFFINEINIPSINYTLRALRLVHVCMAVEPLARIIRVILQSVPDMANIMVLILFFMLVFSVFGVTLFGAFVPKHFQNIQVALYTLFICITQDGWVDIYSDFQTEKREYAMEIGGAIYFTIFITIGAFIGINLFVIVVTTNLEQMMKAGEQGQQQRITFSETGAEEEEENDQLPLVHCVVARSEKSGLLQEPLAGGPLSNLSENTCDNFCLVLEAIQENLRQYKEIRDELNMIVEEVRAIRFNQEQESEVLNRRSSTSGSLETTSSKDIRQMSQQQDLLSALVSMEKVHDSSSQILLKKHKSSH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
88
Horse
88
Sheep
88
Bovine
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte.

細胞の位置付け:

Cell projection>Cilium>Flagellum membrane>Multi-pass membrane protein.
Note: Specifically located in the principal piece of sperm tail.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Testis-specific.

タンパク質ファミリー:

Belongs to the cation channel sperm-associated (TC 1.A.1.19) family.

参考文献

1). The Olfactory Receptor Olfr25 Mediates Sperm Dysfunction Induced by Low-Dose Bisphenol A through the CatSper-Ca2+ Signaling Pathway. Toxics, 2024 (PubMed: 38922122) [IF=4.6]

Application: WB    Species: Mouse    Sample: GC-2 cells

Figure 4 The effect of Olfr25 knockdown on calcium ion levels and the expression of CatSper channel genes in GC-2 cells. (A) RT-qPCR was used to measure the mRNA expression levels of Olfr25 (n = 3). (B) Intracellular calcium ion concentration was detected after Olfr25 knockdown (n = 3). (C) The mRNA expression levels of Catsper1–4 in GC-2 cells after Olfr25 knockdown were detected using qRT-PCR (n = 3). (D) The protein expression levels of CatSper1–4 in GC-2 cells after Olfr25 knockdown were detected using Western blot analyses. (E) Quantitative analysis of the indicated proteins using Image J software. All data are presented as the mean ± SD. ns, not significant. ** p < 0.01.

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