CEACAM7 Antibody - #DF9346

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製品: CEACAM7 Antibody
カタログ: DF9346
タンパク質の説明: Rabbit polyclonal antibody to CEACAM7
アプリケーション: WB IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human
分子量: 29kDa; 29kD(Calculated).
ユニプロット: Q14002
RRID: AB_2842542

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MSDS
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IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
CEACAM7 Antibody detects endogenous levels of total CEACAM7.
RRID:
AB_2842542
引用形式: Affinity Biosciences Cat# DF9346, RRID:AB_2842542.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Carcinoembryonic antigen CGM 2; Carcinoembryonic antigen CGM2; Carcinoembryonic antigen gene family member 2; Carcinoembryonic antigen related cell adhesion molecule 7; Carcinoembryonic antigen-related cell adhesion molecule 7; CEA; CEACAM 7; CEACAM7; CEAM7_HUMAN; CGM 2; CGM2;

免疫原

免疫原:

A synthesized peptide derived from human CEACAM7, corresponding to a region within the internal amino acids.

Uniprot:

Q14002(CEAM7_HUMAN) >>Visit HPA database.

遺伝子(ID):
CEACAM7(1087)
発現特異性:
Q14002 CEAM7_HUMAN:

Expressed in columnar epithelial cells of the colon (at protein level) (PubMed:10436421). Strongly down-regulated in colonic adenocarcinomas.

タンパク質配列:
MGSPSACPYRVCIPWQGLLLTASLLTFWNLPNSAQTNIDVVPFNVAEGKEVLLVVHNESQNLYGYNWYKGERVHANYRIIGYVKNISQENAPGPAHNGRETIYPNGTLLIQNVTHNDAGFYTLHVIKENLVNEEVTRQFYVFSEPPKPSITSNNFNPVENKDIVVLTCQPETQNTTYLWWVNNQSLLVSPRLLLSTDNRTLVLLSATKNDIGPYECEIQNPVGASRSDPVTLNVRYESVQASSPDLSAGTAVSIMIGVLAGMALI

研究背景

細胞の位置付け:

Cell membrane>Lipid-anchor. Apical cell membrane.
Note: Localized to the apical glycocalyx surface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in columnar epithelial cells of the colon (at protein level). Strongly down-regulated in colonic adenocarcinomas.

タンパク質ファミリー:

Belongs to the immunoglobulin superfamily. CEA family.

参考文献

1). RNA-sequencing expression profile and functional analysis of retinal pigment epithelium in atrophic age-related macular degeneration. Journal of biomedical research, 2024 (PubMed: 38808557) [IF=2.2]

Application: WB    Species: human    Sample: ARPE-19 cells

Figure 6. Validation of the ROS-CPXM2-EMT axis in oxidative stress cell models. A: Representative images of Western blotting (WB) analysis of 4-hydroxynonenal (4-HNE) and CPXM2 in ARPE-19 cells treated with or without H2O2 (200 μmol/L), and the relative quantification of 4-HNE and CPXM2. B: Representative immunofluorescence images and mean fluorescence intensity analysis of CPXM2 in ARPE-19 cells treated with H2O2 (200 μmol/L). Scale bar: 50 μm. C: WB analysis of fibronectin 1 (FN1) and SNAIL in ARPE-19 cells treated with H2O2 (200 μmol/L), and the relative quantification of FN1 and SNAIL expression. D: Representative immunofluorescence images and mean fluorescence intensity analysis of ZO-1 and bestrophin-1 (BEST1) in ARPE-19 cells treated with H2O2 (200 μmol/L). Scale bar: 50 μm. E: Quantitative reverse transcription PCR analyses were performed to detect the expression levels of CPXM2 mRNA in ARPE-19 cells transfected with CPXM2 siRNA (siCPXM2). F: Images of WB analysis of ARPE-19 cells transfected with negative siRNA (siNC) or siCPXM2 and stimulated with PBS or H2O2 (200 μmol/L), and the relative quantification analysis of FN1, CPXM2 and αSMA expression. The data are presented as means with standard errors of the mean from three independent experiments. Student's t-test was applied for comparisons between two groups. ANOVA and Tukey's Honestly-Significant Difference post hoc test were applied for comparisons between the three groups

Application: IF/ICC    Species: human    Sample: ARPE-19 cells

Figure 6. Validation of the ROS-CPXM2-EMT axis in oxidative stress cell models. A: Representative images of Western blotting (WB) analysis of 4-hydroxynonenal (4-HNE) and CPXM2 in ARPE-19 cells treated with or without H2O2 (200 μmol/L), and the relative quantification of 4-HNE and CPXM2. B: Representative immunofluorescence images and mean fluorescence intensity analysis of CPXM2 in ARPE-19 cells treated with H2O2 (200 μmol/L). Scale bar: 50 μm. C: WB analysis of fibronectin 1 (FN1) and SNAIL in ARPE-19 cells treated with H2O2 (200 μmol/L), and the relative quantification of FN1 and SNAIL expression. D: Representative immunofluorescence images and mean fluorescence intensity analysis of ZO-1 and bestrophin-1 (BEST1) in ARPE-19 cells treated with H2O2 (200 μmol/L). Scale bar: 50 μm. E: Quantitative reverse transcription PCR analyses were performed to detect the expression levels of CPXM2 mRNA in ARPE-19 cells transfected with CPXM2 siRNA (siCPXM2). F: Images of WB analysis of ARPE-19 cells transfected with negative siRNA (siNC) or siCPXM2 and stimulated with PBS or H2O2 (200 μmol/L), and the relative quantification analysis of FN1, CPXM2 and αSMA expression. The data are presented as means with standard errors of the mean from three independent experiments. Student's t-test was applied for comparisons between two groups. ANOVA and Tukey's Honestly-Significant Difference post hoc test were applied for comparisons between the three groups

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