ARPP19 Antibody - #DF9325

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製品: ARPP19 Antibody
カタログ: DF9325
タンパク質の説明: Rabbit polyclonal antibody to ARPP19
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Rabbit, Dog, Chicken
分子量: 12 kDa(Observed); 12kD(Calculated).
ユニプロット: P56211
RRID: AB_2842521

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#DF9325-BP

ARPP19 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(91%)
クローナリティ:
Polyclonal
特異性:
ARPP19 Antibody detects endogenous levels of total ARPP19.
RRID:
AB_2842521
引用形式: Affinity Biosciences Cat# DF9325, RRID:AB_2842521.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ARP19_HUMAN; ARPP-19; Arpp19; cAMP-regulated phosphoprotein 19;

免疫原

免疫原:

A synthesized peptide derived from human ARPP19, corresponding to a region within N-terminal amino acids.

Uniprot:

P56211(ARP19_HUMAN) >>Visit HPA database.

遺伝子(ID):
ARPP19(10776)
タンパク質配列:
MSAEVPEAASAEEQKEMEDKVTSPEKAEEAKLKARYPHLGQKPGGSDFLRKRLQKGQKYFDSGDYNMAKAKMKNKQLPTAAPDKTEVTGDHIPTPQDLPQRKPSLVASKLAG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Chicken
91
Xenopus
73
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-62 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. May indirectly enhance GAP-43 expression.

PTMs:

Phosphorylation at Ser-62 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A (By similarity). Phosphorylated by PKA.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the endosulfine family.

参考文献

1). LncRNA XIST from the bone marrow mesenchymal stem cell derived exosome promotes osteosarcoma growth and metastasis through miR-655/ACLY signal. Cancer cell international, 2022 (PubMed: 36309693) [IF=5.8]

Application: WB    Species: human    Sample: BMSCs

Fig. 6 BMSCs derived exosomal XIST regulated ACLY expression in osteosarcoma cells by binding miR-655. A Western blot analysis the effect of BMSCs derived exosomal XIST combined with miR-655 on the expression of ARPP19, TOB1 and ACLY in osteosarcoma cells (n = 3); B At animal level, western blot analysis the effect of BMSCs derived exosomal XIST combined with miR-655 on ACLY expression (n = 3); C The binding pattern of miR-655 and the ACLY 3'-UTR and the corresponding mutation information; D The binding of miR-655 and the ACLY 3'-UTR and their possible sites analyzed by dual luciferase reporter gene assay (n = 3). ** represents p 

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