製品: CNN3 Antibody
カタログ: DF9323
タンパク質の説明: Rabbit polyclonal antibody to CNN3
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat, Monkey
予測: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 36 kDa(Observed); 36kD(Calculated).
ユニプロット: Q15417
RRID: AB_2842519

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat, Monkey
予測:
Bovine(100%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
クローナリティ:
Polyclonal
特異性:
CNN3 Antibody detects endogenous levels of total CNN3.
RRID:
AB_2842519
引用形式: Affinity Biosciences Cat# DF9323, RRID:AB_2842519.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Acidic calponin 3; acidic isoform; Calponin 3 acidic; Calponin 3; Calponin acidic; Calponin acidic isoform; Calponin; Calponin-3; Calponin3; CNN 3; Cnn3; CNN3_HUMAN; dJ639P13.2.2; OTTHUMP00000012470; OTTHUMP00000012471;

免疫原

免疫原:

A synthesized peptide derived from human CNN3, corresponding to a region within C-terminal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
Q15417 CNN3_HUMAN:

Expressed in both non-smooth muscle tissues as well as smooth muscle tissues.

タンパク質配列:
MTHFNKGPSYGLSAEVKNKIASKYDHQAEEDLRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDLFENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMGTNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMLAPGTRRDIYDQKLTLQPVDNSTISLQMGTNKVASQKGMSVYGLGRQVYDPKYCAAPTEPVIHNGSQGTGTNGSEISDSDYQAEYPDEYHGEYQDDYPRDYQYSDQGIDY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Sheep
92
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

組織特異性:

Expressed in both non-smooth muscle tissues as well as smooth muscle tissues.

タンパク質ファミリー:

Belongs to the calponin family.

参考文献

1). Promoting effects of calponin 3 on the growth of diffuse large B‑cell lymphoma cells. Oncology Reports, 2023 (PubMed: 36660952) [IF=3.8]

Application: WB    Species: Human    Sample: DLBCL cells

Figure 2. - Overexpression and knockdown of CNN3 in DLBCL cells. The changes in the expression of CNN3 in DB and SU-DHL-4 cells were confirmed using (A and B) reverse transcription-quantitative PCR and (C) western blot analysis following transfection with CNN3 overexpression vector or siRNA. ***P

2). The modulation of lung cancer cell motility by Calponin 3 is achieved through its ability to sense and respond to changes in substrate stiffness. Biotechnology and bioengineering, 2024 (PubMed: 39279163) [IF=3.5]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.