BATF2 Antibody - #DF9266

(1)   (2)  
製品: BATF2 Antibody
カタログ: DF9266
タンパク質の説明: Rabbit polyclonal antibody to BATF2
アプリケーション: WB
Cited expt.: WB
反応性: Human
予測: Pig, Horse, Sheep, Dog
分子量: 29kDa; 29kD(Calculated).
ユニプロット: Q8N1L9
RRID: AB_2842462

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MS Report
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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(91%), Horse(82%), Sheep(91%), Dog(90%)
クローナリティ:
Polyclonal
特異性:
BATF2 Antibody detects endogenous levels of total BATF2.
RRID:
AB_2842462
引用形式: Affinity Biosciences Cat# DF9266, RRID:AB_2842462.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

B ATF 2; Basic leucine zipper transcriptional factor ATF like 2; BATF2; MGC20410; SARI; Suppressor of AP 1 regulated by IFN;

免疫原

免疫原:

A synthesized peptide derived from human BATF2, corresponding to a region within the internal amino acids.

Uniprot:

Q8N1L9(BATF2_HUMAN) >>Visit HPA database.

遺伝子(ID):
BATF2(116071)
タンパク質配列:
MHLCGGNGLLTQTDPKEQQRQLKKQKNRAAAQRSRQKHTDKADALHQQHESLEKDNLALRKEIQSLQAELAWWSRTLHVHERLCPMDCASCSAPGLLGCWDQAEGLLGPGPQGQHGCREQLELFQTPGSCYPAQPLSPGPQPHDSPSLLQCPLPSLSLGPAVVAEPPVQLSPSPLLFASHTGSSLQGSSSKLSALQPSLTAQTAPPQPLELEHPTRGKLGSSPDNPSSALGLARLQSREHKPALSAATWQGLVVDPSPHPLLAFPLLSSAQVHF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Sheep
91
Dog
90
Horse
82
Rabbit
73
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system. Following infection, participates in the differentiation of CD8(+) thymic conventional dendritic cells in the immune system. Acts via the formation of a heterodimer with JUN family proteins that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3' and regulates expression of target genes (By similarity). Selectively suppresses CCN1 transcription and hence blocks the downstream cell proliferation signals produced by CCN1 and inhibits CCN1-induced anchorage-independent growth and invasion in several cancer types, such as breast cancer, malignant glioma and metastatic melanoma. Possibly acts by interfering with AP-1 binding to CCN1 promoter.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the bZIP family.

参考文献

1). Circ_0005927 Inhibits the Progression of Colorectal Cancer by Regulating miR-942-5p/BATF2 Axis. Cancer Management and Research, 2021 (PubMed: 33732022) [IF=2.5]

Application: WB    Species: Human    Sample: NCM460, LoVo and SW620 cells

Figure 5 Circ_0005927 sponged miR-942-5p to regulate BATF2. (A) The binding sites between miR-942-5p and BATF2 were predicted by TargetScan online database. (B and C) Dual-luciferase reporter assay was performed to verify that miR-942-5p bound to BATF2. (D) Western blot was carried out to detect the protein expression of BATF2 in NCM460, LoVo and SW620 cells. (E) QRT-PCR was employed to determine BATF2 mRNA expression in CRC tissues and normal intestinal tissues. (F and G) The linear relationship between BATF2 expression and circ_0005927 expression or miR-942-5p expression was explained by Spearman correlation analysis. (H) The interfering efficiency of anti-miR-942-5p was determined by qRT-PCR. (I) The effect of miR-942-5p inhibitor on the protein expression of BATF2 was disclosed by Western blot. (J) The impacts between circ_0005927 overexpression and miR-942-5p mimic on BATF2 protein expression were unveiled by Western blot. **P<0.01.

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