DDX18 Antibody - #DF9258

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製品: DDX18 Antibody
カタログ: DF9258
タンパク質の説明: Rabbit polyclonal antibody to DDX18
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human
予測: Bovine, Sheep
分子量: 75kDa; 75kD(Calculated).
ユニプロット: Q9NVP1
RRID: AB_2842454

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Bovine(89%), Sheep(89%)
クローナリティ:
Polyclonal
特異性:
DDX18 Antibody detects endogenous levels of total DDX18.
RRID:
AB_2842454
引用形式: Affinity Biosciences Cat# DF9258, RRID:AB_2842454.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ATP dependent RNA helicase DDX18; ATP-dependent RNA helicase DDX18; DDX 18; DDX18; DDX18_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 18; DEAD box polypeptide 18; DEAD box protein 18; FLJ33908; MrDb; Myc regulated DEAD box protein; Myc-regulated DEAD box protein;

免疫原

免疫原:

A synthesized peptide derived from human DDX18, corresponding to a region within N-terminal amino acids.

Uniprot:

Q9NVP1(DDX18_HUMAN) >>Visit HPA database.

遺伝子(ID):
DDX18(8886)
タンパク質配列:
MSHLPMKLLRKKIEKRNLKLRQRNLKFQGASNLTLSETQNGDVSEETMGSRKVKKSKQKPMNVGLSETQNGGMSQEAVGNIKVTKSPQKSTVLTNGEAAMQSSNSESKKKKKKKRKMVNDAEPDTKKAKTENKGKSEEESAETTKETENNVEKPDNDEDESEVPSLPLGLTGAFEDTSFASLCNLVNENTLKAIKEMGFTNMTEIQHKSIRPLLEGRDLLAAAKTGSGKTLAFLIPAVELIVKLRFMPRNGTGVLILSPTRELAMQTFGVLKELMTHHVHTYGLIMGGSNRSAEAQKLGNGINIIVATPGRLLDHMQNTPGFMYKNLQCLVIDEADRILDVGFEEELKQIIKLLPTRRQTMLFSATQTRKVEDLARISLKKEPLYVGVDDDKANATVDGLEQGYVVCPSEKRFLLLFTFLKKNRKKKLMVFFSSCMSVKYHYELLNYIDLPVLAIHGKQKQNKRTTTFFQFCNADSGTLLCTDVAARGLDIPEVDWIVQYDPPDDPKEYIHRVGRTARGLNGRGHALLILRPEELGFLRYLKQSKVPLSEFDFSWSKISDIQSQLEKLIEKNYFLHKSAQEAYKSYIRAYDSHSLKQIFNVNNLNLPQVALSFGFKVPPFVDLNVNSNEGKQKKRGGGGGFGYQKTKKVEKSKIFKHISKKSSDSRQFSH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
89
Sheep
89
Horse
75
Xenopus
67
Dog
63
Rabbit
63
Pig
58
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Probable RNA-dependent helicase.

細胞の位置付け:

Nucleus>Nucleolus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.

参考文献

1). DDX18 Facilitates the Tumorigenesis of Lung Adenocarcinoma by Promoting Cell Cycle Progression through the Upregulation of CDK4. International journal of molecular sciences, 2024 (PubMed: 38732173) [IF=5.6]

Application: WB    Species: Human    Sample: H157 and H460 cells

Figure 2 DDX18 Upregulation in LUAD Tissues and its Functional Role in LUAD Cells. (a,b) Heatmaps depicting global mRNA expression profiles in GSE75037 [(a) non-tumor tissues (n = 83), LUAD tissues (n = 83)] and TCGA [(b) normal lung tissues (n = 287), LUAD tissues (n = 574)] datasets. DDX18 expression is indicated by the white line. (c,d) Boxplots representing DDX18 mRNA expression levels in non-tumor and LUAD tissues from GSE75037 (c) and TCGA (d) datasets. p-values are indicated. (e) Kaplan–Meier survival curve for overall survival of LUAD patients with high (n = 126) and low (n = 376) DDX18 expression. Low DDX18 expression correlates with improved overall survival (p-value indicated). (f) Relative DDX18 mRNA levels in H157 (n = 3) and H460 (n = 4) cells following DDX18 knockdown. Data represent mean ± SD. (g) Western blot analysis confirming DDX18 knockdown efficiency in H157 and H460 cells. (h) DDX18 knockdown significantly suppresses cell proliferation in H157 and H460 cells (n = 3, mean ± SD). (i) DDX18 knockdown substantially reduces colony formation capacity in H157 and H460 cells (n = 3, mean ± SD). (j) DDX18 knockdown inhibits the migration of H157 and H460 cells in vitro (n = 3, mean ± SD). ** p < 0.01; *** p < 0.001; **** p < 0.0001. LUAD, lung adenocarcinoma; SC, scramble control; KD, knockdown.

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