AQP9 Antibody - #DF9225

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製品: AQP9 Antibody
カタログ: DF9225
タンパク質の説明: Rabbit polyclonal antibody to AQP9
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 31 kDa(Observed); 31kD(Calculated).
ユニプロット: O43315
RRID: AB_2842421

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#DF9225-BP

AQP9 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(83%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
クローナリティ:
Polyclonal
特異性:
AQP9 Antibody detects endogenous levels of total AQP9.
RRID:
AB_2842421
引用形式: Affinity Biosciences Cat# DF9225, RRID:AB_2842421.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AQP 9; AQP-9; Aqp9; AQP9_HUMAN; Aquaglyceroporin-9; Aquaporin-9; Aquaporin9; HsT 17287; HsT17287; Small solute channel 1; SSC 1; SSC1;

免疫原

免疫原:

A synthesized peptide derived from human AQP9, corresponding to a region within the internal amino acids.

Uniprot:

O43315(AQP9_HUMAN) >>Visit HPA database.

遺伝子(ID):
AQP9(366)
発現特異性:
O43315 AQP9_HUMAN:

Highly expressed in peripheral leukocytes. Also expressed in liver, lung, and spleen.

タンパク質配列:
MQPEGAEKGKSFKQRLVLKSSLAKETLSEFLGTFILIVLGCGCVAQAILSRGRFGGVITINVGFSMAVAMAIYVAGGVSGGHINPAVSLAMCLFGRMKWFKLPFYVGAQFLGAFVGAATVFGIYYDGLMSFAGGKLLIVGENATAHIFATYPAPYLSLANAFADQVVATMILLIIVFAIFDSRNLGAPRGLEPIAIGLLIIVIASSLGLNSGCAMNPARDLSPRLFTALAGWGFEVFRAGNNFWWIPVVGPLVGAVIGGLIYVLVIEIHHPEPDSVFKTEQSEDKPEKYELSVIM

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Horse
83
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Forms a water channel with a broad specificity. Also permeable glycerol and urea. Mediates passage of a wide variety of small, non-charged solutes including carbamides, polyols, purines, and pyrimidines.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in peripheral leukocytes. Also expressed in liver, lung, and spleen.

タンパク質ファミリー:

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Belongs to the MIP/aquaporin (TC 1.A.8) family.

参考文献

1). LncRNA-mRNA co-expression analysis reveals aquaporin-9-promoted neutrophil extracellular trap formation and inflammatory activation in sepsis. International immunopharmacology, 2024 (PubMed: 39133961) [IF=4.8]
2). Comparison of the effect of raw and prepared Atractylodis Macrocephalae Rhizoma - Atractylodis Rhizoma couplet medicines on strengthening the spleen and transforming dampness. Journal of ethnopharmacology, 2026 (PubMed: 41638459) [IF=4.8]
3). Sodium tanshinone IIA sulfonate alleviates fetal growth restriction by mediating aquaporin-3 expression in placental trophoblast cells. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2025 (PubMed: 39825721) [IF=4.4]
4). D-Carvone Mitigating Renal Damage and Enhancing Antioxidant Defense in Lithium Induced Nephrotoxicity. Food science & nutrition, 2025 (PubMed: 41019175) [IF=3.5]
5). Tanshinone IIA increased amniotic fluid volume through down-regulating placental AQPs expression via inhibiting the activity of GSK-3β. CELL AND TISSUE RESEARCH, 2022 (PubMed: 35674921) [IF=3.2]
6). Arctiin alleviates functional constipation by enhancing intestinal motility in mice. Experimental and Therapeutic Medicine, 2023 (PubMed: 37090075) [IF=2.4]

Application: IF/ICC    Species: Mouse    Sample: colon tissue

Figure 5 Arc reverses loperamide-induced elevation of AQP-3 and AQP-9. Immunofluorescence analysis of (A) AQP-3 and (B) AQP-9 in colon tissue. (C) Evaluation of immunofluorescent staining was performed using Image-Pro Plus software. (D) Western blot analysis of protein expression levels of AQP-3 and AQP-9. (E) Densitometry analysis of western blots. **P

Application: WB    Species: Mouse    Sample: colon tissue

Figure 5 Arc reverses loperamide-induced elevation of AQP-3 and AQP-9. Immunofluorescence analysis of (A) AQP-3 and (B) AQP-9 in colon tissue. (C) Evaluation of immunofluorescent staining was performed using Image-Pro Plus software. (D) Western blot analysis of protein expression levels of AQP-3 and AQP-9. (E) Densitometry analysis of western blots. **P

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