ADAM33 Antibody - #DF9166

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製品: ADAM33 Antibody
カタログ: DF9166
タンパク質の説明: Rabbit polyclonal antibody to ADAM33
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC
反応性: Human, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 88 kDa(Observed); 88kD(Calculated).
ユニプロット: Q9BZ11
RRID: AB_2842362

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#DF9166-BP

ADAM33 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Rat
予測:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Xenopus(%)
クローナリティ:
Polyclonal
特異性:
ADAM33 Antibody detects endogenous levels of total ADAM33.
RRID:
AB_2842362
引用形式: Affinity Biosciences Cat# DF9166, RRID:AB_2842362.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

A disintegrin and metalloprotease 33; A disintegrin and metalloproteinase domain 33; ADA33_HUMAN; ADAM 33; ADAM metallopeptidase domain 33; ADAM33; C20orf153; Disintegrin and metalloproteinase domain-containing protein 33; Disintegrin and reprolysin metalloproteinase family protein; DJ964F7.1; DKFZp434K0521; FLJ35308; FLJ36751; Metalloprotease disintegrin; MGC149823; MGC71889; PRO1891; UNQ873;

免疫原

免疫原:

A synthesized peptide derived from human ADAM33, corresponding to a region within the internal amino acids.

Uniprot:

Q9BZ11(ADA33_HUMAN) >>Visit HPA database.

遺伝子(ID):
ADAM33(80332)
発現特異性:
Q9BZ11 ADA33_HUMAN:

Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins.

タンパク質配列:
MGWRPRRARGTPLLLLLLLLLLWPVPGAGVLQGHIPGQPVTPHWVLDGQPWRTVSLEEPVSKPDMGLVALEAEGQELLLELEKNHRLLAPGYIETHYGPDGQPVVLAPNHTDHCHYQGRVRGFPDSWVVLCTCSGMSGLITLSRNASYYLRPWPPRGSKDFSTHEIFRMEQLLTWKGTCGHRDPGNKAGMTSLPGGPQSRGRREARRTRKYLELYIVADHTLFLTRHRNLNHTKQRLLEVANYVDQLLRTLDIQVALTGLEVWTERDRSRVTQDANATLWAFLQWRRGLWAQRPHDSAQLLTGRAFQGATVGLAPVEGMCRAESSGGVSTDHSELPIGAAATMAHEIGHSLGLSHDPDGCCVEAAAESGGCVMAAATGHPFPRVFSACSRRQLRAFFRKGGGACLSNAPDPGLPVPPALCGNGFVEAGEECDCGPGQECRDLCCFAHNCSLRPGAQCAHGDCCVRCLLKPAGALCRQAMGDCDLPEFCTGTSSHCPPDVYLLDGSPCARGSGYCWDGACPTLEQQCQQLWGPGSHPAPEACFQVVNSAGDAHGNCGQDSEGHFLPCAGRDALCGKLQCQGGKPSLLAPHMVPVDSTVHLDGQEVTCRGALALPSAQLDLLGLGLVEPGTQCGPRMVCQSRRCRKNAFQELQRCLTACHSHGVCNSNHNCHCAPGWAPPFCDKPGFGGSMDSGPVQAENHDTFLLAMLLSVLLPLLPGAGLAWCCYRLPGAHLQRCSWGCRRDPACSGPKDGPHRDHPLGGVHPMELGPTATGQPWPLDPENSHEPSSHPEKPLPAVSPDPQADQVQMPRSCLW

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Bovine
91
Sheep
91
Rabbit
91
Xenopus
82
Chicken
73
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

PTMs:

The precursor is cleaved by a furin endopeptidase.

細胞の位置付け:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins.

タンパク質ファミリー:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

参考文献

1). Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling. Molecular Medicine Reports, 2021 (PubMed: 33982767) [IF=3.4]

Application: IHC    Species: Human    Sample: lung tissue

Figure 2. Immunohistochemical staining of ADAM33 in lung tissue of a patient with asthma. (A) Representative images showed that ADAM33 was mainly expressed in epithelial cells, smooth muscle cells, endothelial cells and interstitial inflammatory cells in the lung tissue of asthmatic patients. Scale bar, 200 µm. (B) Representative images showed that ADAM33 was also expressed in airway VSMCs in the lung tissue of asthmatic patients. Arrows indicate ADAM33 stained in brown in airway VSMCs in the lung tissue of asthmatic patients. Scale bar, 100 µm. ADAM33, a disintegrin and metalloproteinase-33; VSMCs, vascular smooth muscle cells.

Application: WB    Species: Human    Sample: HASMCs

Figure 4. Validation of lentivirus silencing effect of ADAM33 in HASMCs. (A) Comparison of ADAM33 mRNA levels among HASMCs in groups of blank control (no treatment), LV-NC and ADAM33 silencing with LV-ADAM33-shRNA 1–3 with reverse transcription-quantitative PCR. (B) Comparison of ADAM33 protein levels among HASMCs in groups of blank control (no treatment), LV-NC and ADAM33 silencing with LV-ADAM33-shRNA with western blotting. (C) Semi-quantitative analysis for the ADAM33 protein levels among HASMCs in blank control, LV-NC and LV-ADAM33-shRNA groups. *P<0.05. ADAM33, a disintegrin and metalloproteinase-33; HASMCs, human aortic smooth muscle cells; LV, lentiviral vector; NC, negative control; shRNA, short-hairpin RNA.

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