製品: AOAH Antibody
カタログ: DF9158
タンパク質の説明: Rabbit polyclonal antibody to AOAH
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 65 kDa(Observed); 65kD(Calculated).
ユニプロット: P28039
RRID: AB_2842354

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
予測:
Pig(83%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
クローナリティ:
Polyclonal
特異性:
AOAH Antibody detects endogenous levels of total AOAH.
RRID:
AB_2842354
引用形式: Affinity Biosciences Cat# DF9158, RRID:AB_2842354.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Acyloxyacyl hydrolase (neutrophil); Acyloxyacyl hydrolase large subunit; AOAH; AOAH_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human AOAH, corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
タンパク質配列:
MQSPWKILTVAPLFLLLSLQSSASPANDDQSRPSLSNGHTCVGCVLVVSVIEQLAQVHNSTVQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKLLSADMNADVVCHTLEFCKQNTGQPLCHLYPLPKETWKFTLQKARQIVKKSPILKYSRSGSDICSLPVLAKICQKIKLAMEQSVPFKDVDSDKYSVFPTLRGYHWRGRDCNDSDESVYPGRRPNNWDVHQDSNCNGIWGVDPKDGVPYEKKFCEGSQPRGIILLGDSAGAHFHISPEWITASQMSLNSFINLPTALTNELDWPQLSGATGFLDSTVGIKEKSIYLRLWKRNHCNHRDYQNISRNGASSRNLKKFIESLSRNKVLDYPAIVIYAMIGNDVCSGKSDPVPAMTTPEKLYSNVMQTLKHLNSHLPNGSHVILYGLPDGTFLWDNLHNRYHPLGQLNKDMTYAQLYSFLNCLQVSPCHGWMSSNKTLRTLTSERAEQLSNTLKKIAASEKFTNFNLFYMDFAFHEIIQEWQKRGGQPWQLIEPVDGFHPNEVALLLLADHFWKKVQLQWPQILGKENPFNPQIKQVFGDQGGH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
92
Dog
92
Pig
83
Horse
83
Sheep
83
Rabbit
83
Xenopus
75
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses (By similarity). In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria (By similarity).

PTMs:

Cleaved into a large and a small subunit.

The small subunit is N-glycosylated.

細胞の位置付け:

Secreted. Cytoplasmic vesicle.
Note: Detected in urine.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

参考文献

1). Proteomic analysis exploring the mechanism of bladder fibrosis induced by ketamine using a rat model. Translational andrology and urology, 2021 (PubMed: 34532254) [IF=1.9]

Application: WB    Species: Rat    Sample:

Figure 4. Proteomics results verification by RT-PCR and WB. (A) Verification results of the 10 selected mRNA. The expression of DNase I, LBDH2, LOXHD1, FCGBP, DMN, GIPC3, PHACTR3, Hba-a3, and Mcpt2 were consistent with the proteomics results. (B) Representative images of western blot analysis examining AOAH and MC protease 1 expression in rat bladders. The expression of AOAH and MC protease 1 in the experimental group decreased compared to the control group. (C) Quantitative densitometric analysis of the ratio of AOAH versus GAPDH loading control. The experimental group was significantly reduced, P=0.0117. (D) Quantitative densitometric analysis of the ratio of MC protease 1 versus GAPDH loading control. The experimental group was significantly reduced, at P=0.0056. Results are expressed as the mean ± SEM. *, P

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For Research Use Only.
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