SP6 Antibody - #DF9082

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製品: SP6 Antibody
カタログ: DF9082
タンパク質の説明: Rabbit polyclonal antibody to SP6
アプリケーション: WB IHC
Cited expt.:
反応性: Human, Mouse, Monkey
予測: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog
分子量: 40 kDa; 40kD(Calculated).
ユニプロット: Q3SY56
RRID: AB_2842278

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関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Monkey
予測:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
SP6 Antibody detects endogenous levels of total SP6.
RRID:
AB_2842278
引用形式: Affinity Biosciences Cat# DF9082, RRID:AB_2842278.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

MGC119665; Epfn; EPIPROFIN; KLF14; Krueppel-like factor 14; MGC119662; MGC119663; MGC119664; Sp6; Sp6 transcription factor; SP6_HUMAN; Transcription factor Sp6;

免疫原

免疫原:

A synthesized peptide derived from human SP6, corresponding to a region within the internal amino acids.

Uniprot:

Q3SY56(SP6_HUMAN) >>Visit HPA database.

遺伝子(ID):
SP6(80320)
発現特異性:
Q3SY56 SP6_HUMAN:

Ubiquitous.

タンパク質配列:
MLTAVCGSLGSQHTEAPHASPPRLDLQPLQTYQGHTSPEAGDYPSPLQPGELQSLPLGPEVDFSQGYELPGASSRVTCEDLESDSPLAPGPFSKLLQPDMSHHYESWFRPTHPGAEDGSWWDLHPGTSWMDLPHTQGALTSPGHPGALQAGLGGYVGDHQLCAPPPHPHAHHLLPAAGGQHLLGPPDGAKALEVAAPESQGLDSSLDGAARPKGSRRSVPRSSGQTVCRCPNCLEAERLGAPCGPDGGKKKHLHNCHIPGCGKAYAKTSHLKAHLRWHSGDRPFVCNWLFCGKRFTRSDELQRHLQTHTGTKKFPCAVCSRVFMRSDHLAKHMKTHEGAKEEAAGAASGEGKAGGAVEPPGGKGKREAEGSVAPSN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Zebrafish
80
Sheep
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Promotes cell proliferation.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous.

タンパク質ファミリー:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the Sp1 C2H2-type zinc-finger protein family.

参考文献

1). FOXO4-SP6 axis controls surface epithelium commitment by mediating epigenomic remodeling. Stem cell reports, 2025 (PubMed: 40086444) [IF=5.9]

Application: IF/ICC    Species: human    Sample:

Figure 4 SP6 depletion impairs SE commitment (A) Genome browser view of FOXO4, H3K4me3, and RNA-seq signal across SP6 loci. (B) qRT-PCR analysis for expression level of SP6 in scrambled shRNA- and shFOXO4-treated cells on D3 and D7. qRT-PCR values were normalized to the values in scrambled shRNA group. Values are shown as means ± SD (n = 3 independent experiments; ∗∗∗p < 0.001 t test). (C) qRT-PCR analysis for expression level of FOXO4 in scrambled shRNA- and shSP6-treated differentiated cells on D3. qRT-PCR values were normalized to the values in scrambled shRNA group. Values are shown as means ± SD (n = 3 independent experiments; ns means not statistically significant, t test). (D) Immunostaining for SP6 in hESCs and differentiated cells on D3. Scale bar, 100 μm. (E) Phase-contrast images and immunostaining for KRT18 and TP63 in scrambled shRNA- and shSP6-treated differentiated cells on D7. Scale bar, 100 μm. (F) qRT-PCR analysis for expression levels of representative genes of SE hallmark and regulators in scrambled shRNA- and shSP6-treated differentiated cells on D3. qRT-PCR values were normalized to the values in scrambled shRNA group. Values are shown as means ± SD (n = 3 independent experiments; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 t test). (G) GSEA for SE identity genes in the gene expression matrix of shSP6- versus scrambled shRNA-treated differentiated cells on D3 and D7. The used SE identity gene set is from published research (Huang et al., 2022). (H) GO (biological process) analysis of the up-regulated (left) and down-regulated genes (right) upon SP6 knockdown. (I) GSEA for down-regulated genes upon SP6 knockdown in the gene expression matrix of shFOXO4- versus scrambled shRNA-treated differentiated cells on D3 and D7.

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