GOLT1B Antibody - #DF9071

Fig. 2 GOLT1B promotes CRC cell proliferation, invasion and migration. a, b GOLT1B mRNA and protein level were determined by qPCR and western blot in HCT116 and RKO cells. c, d Cell migration were determined by wound healing experiment after siRNA transfection or overexpression of GOLT1B, respectively. e, f Cell invasion were detected by transwell assays with matrigel after siRNA transfection or overexpression of GOLT1B, respectively. g Effect of GOLT1B on proliferation of colorectal cancer cells in vitro. All data are shown as mean ± S.E.M. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Fig. 3 GOLT1B promotes metastasis of colorectal cancer in vivo. a GOLT1B stable overexpression was verified by qPCR and western blot. b The migration and invasion ability of HCT116 cells stablely transfected with control vector or GOLT1B were determined by transwell assays in vitro. c The average bioluminescence imaging signals in mice bearing liver metastatic burden was determined by IVIS imaging (HCT116Vector vs. HCT116GOLT1B, n = 8). d Body weight curves of mice in two groups. e Representative images of metastatic foci (pointed out with red arrow) in mice liver. f The number of liver metastatic foci in control vector and GOLT1B group. g IHC staining of GOLT1B and H&E staining in liver metastatic loci

Fig. 5 GOLT1B interacts with DVL2 to promote it transport and localization to the cell membrane. a The nucleus and cytoplasm separation experiment showed that GOLT1B overexpression increased the level of nuclear β-catenin. b The cell membrane and cytoplasm separation experiment showed that GOLT1B could induce DVL2 upregualtion on the cell membrane. c CO-IP experiment confirmed the interaction between GOLT1B and DVL2. d Immunofluorescence experiment showed that GOLT1B and DVL2 were co-localized

Fig. 2 |GOLT1B promotes CRC cell proliferation, invasion and migration. a, b GOLT1B mRNA and protein level were determined by qPCR and western blot in HCT116 and RKO cells.

Figure 2GOLT1B enhances CESC cell proliferation and migration in vitro and in vivo. (A) CCK-8 analysis of cell viability after GOLT1B down- and upregulation. (B) Representation of colony formation assay and its analysis of GOLT1B knockdown/overexpressed SiHa and HeLa cells. Scale bar = 1 cm. (C) Representative EdU staining and quantification analysis after GOLT1B downregulation in SiHa and HeLa cells. Scale bar = 50 µm. (D) Apoptosis assay staining the effect of siNC/siGOLT1B SiHa and HeLa cells. (E-F) Representative image of transwell assay and analysis of GOLT1B knockdown/overexpressed SiHa and HeLa cells. (G) Images of subcutaneously injected shNC/shGOLT1B HeLa cells formed tumors, tumor weight comparison, and growth curve. Representation of H&E staining, Ki67 staining, and GOLT1B staining of a subcutaneous tumor section from BALB/c nude mice. Data were presented as mean ± SD. Two-tailed Student’s t-test. *P