NKAP Antibody - #DF9069

Fig. 6. miR-181a-5p inhibits HK-2 cell senescence through NF-κB pathway. (a) Heatmap of down-regulated miRNA genes in kidney tissues of naturally aging mice (OLD, 24 months old) with young mice (YNG, 8 weeks old) as control. The colour key from blue to red represents scaled expression values from low to high. (b) qRT-PCR of relative levels of miR-181a-5p and miR-181a-1–3p in kidney tissues of OLD and YNG, with the level in YNG group arbitrarily set to 1. (c) qRT-PCR of relative levels of miR-181a-5p and miR-181a-3p in HK-2 cells treated with or without D-gal (200 mmol/L, 5days), with the level in control group arbitrarily set to 1. (d) qRT-PCR of relative levels of miR-181a-5p in HK-2 cells transfected with miRNA-181a-5p mimic or its blank control (NCm), with the level in NCm group arbitrarily set to 1. (e) qRT-PCR of relative mRNA levels of NF-κB and p21 in HK-2 cells transfected with miRNA-181a-5p mimic or its blank control (NCm), with the level in NCm group arbitrarily set to 1. (f-h) Representative western blots of the protein levels of NF-κB, NKAP, and p21 expression in HK-2 cells transfected with miRNA-181a-5p mimic or its blank control (NCm) and miRNA-181a-5p inhibitor or its blank control (NCi), and the quantifications, with the level in NCm (g) or NCi (h) group arbitrarily set to 1. * p