製品: ACINU Antibody
カタログ: DF9051
タンパク質の説明: Rabbit polyclonal antibody to ACINU
アプリケーション: WB
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 152 kDa; 152kD(Calculated).
ユニプロット: Q9UKV3
RRID: AB_2842247

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
ACINU Antibody detects endogenous levels of total ACINU.
RRID:
AB_2842247
引用形式: Affinity Biosciences Cat# DF9051, RRID:AB_2842247.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

ACIN 1; ACIN1; ACINU_HUMAN; Acinus; ACN; Apoptotic chromatin condensation inducer 1; Apoptotic chromatin condensation inducer in the nucleus; DKFZp667N107; fSAP152; functional spliceosome-associated protein 152; KIAA0670;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q9UKV3 ACINU_HUMAN:

Ubiquitous. The Ser-1180 phosphorylated form (by SRPK2) is highly expressed and phosphorylated in patients with myeloid hematologic malignancies.

タンパク質配列:
MWRRKHPRTSGGTRGVLSGNRGVEYGSGRGHLGTFEGRWRKLPKMPEAVGTDPSTSRKMAELEEVTLDGKPLQALRVTDLKAALEQRGLAKSGQKSALVKRLKGALMLENLQKHSTPHAAFQPNSQIGEEMSQNSFIKQYLEKQQELLRQRLEREAREAAELEEASAESEDEMIHPEGVASLLPPDFQSSLERPELELSRHSPRKSSSISEEKGDSDDEKPRKGERRSSRVRQARAAKLSEGSQPAEEEEDQETPSRNLRVRADRNLKTEEEEEEEEEEEEDDEEEEGDDEGQKSREAPILKEFKEEGEEIPRVKPEEMMDERPKTRSQEQEVLERGGRFTRSQEEARKSHLARQQQEKEMKTTSPLEEEEREIKSSQGLKEKSKSPSPPRLTEDRKKASLVALPEQTASEEETPPPLLTKEASSPPPHPQLHSEEEIEPMEGPAPAVLIQLSPPNTDADTRELLVSQHTVQLVGGLSPLSSPSDTKAESPAEKVPEESVLPLVQKSTLADYSAQKDLEPESDRSAQPLPLKIEELALAKGITEECLKQPSLEQKEGRRASHTLLPSHRLKQSADSSSSRSSSSSSSSSRSRSRSPDSSGSRSHSPLRSKQRDVAQARTHANPRGRPKMGSRSTSESRSRSRSRSRSASSNSRKSLSPGVSRDSSTSYTETKDPSSGQEVATPPVPQLQVCEPKERTSTSSSSVQARRLSQPESAEKHVTQRLQPERGSPKKCEAEEAEPPAATQPQTSETQTSHLPESERIHHTVEEKEEVTMDTSENRPENDVPEPPMPIADQVSNDDRPEGSVEDEEKKESSLPKSFKRKISVVSATKGVPAGNSDTEGGQPGRKRRWGASTATTQKKPSISITTESLKSLIPDIKPLAGQEAVVDLHADDSRISEDETERNGDDGTHDKGLKICRTVTQVVPAEGQENGQREEEEEEKEPEAEPPVPPQVSVEVALPPPAEHEVKKVTLGDTLTRRSISQQKSGVSITIDDPVRTAQVPSPPRGKISNIVHISNLVRPFTLGQLKELLGRTGTLVEEAFWIDKIKSHCFVTYSTVEEAVATRTALHGVKWPQSNPKFLCADYAEQDELDYHRGLLVDRPSETKTEEQGIPRPLHPPPPPPVQPPQHPRAEQREQERAVREQWAEREREMERRERTRSEREWDRDKVREGPRSRSRSRDRRRKERAKSKEKKSEKKEKAQEEPPAKLLDDLFRKTKAAPCIYWLPLTDSQIVQKEAERAERAKEREKRRKEQEEEEQKEREKEAERERNRQLEREKRREHSRERDRERERERERDRGDRDRDRERDRERGRERDRRDTKRHSRSRSRSTPVRDRGGRR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Bovine
92
Sheep
92
Xenopus
67
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UKV3 基板として

Site PTM Type Enzyme
K5 Ubiquitination
R8 Methylation
R14 Methylation
S18 Phosphorylation
R29 Methylation
K81 Methylation
K81 Ubiquitination
R87 Methylation
S96 Phosphorylation
K103 Methylation
K103 Ubiquitination
K113 Methylation
S115 Phosphorylation
T116 Phosphorylation
S125 Phosphorylation
S132 Phosphorylation
S135 Phosphorylation
K138 Ubiquitination
K143 Methylation
K143 Ubiquitination
S166 Phosphorylation
S169 Phosphorylation
S189 Phosphorylation
S190 Phosphorylation
S199 Phosphorylation
S206 Phosphorylation
S208 Phosphorylation
S210 Phosphorylation
S216 Phosphorylation
S228 Phosphorylation
S229 Phosphorylation
K238 Acetylation
S240 Phosphorylation
S243 Phosphorylation
T254 Phosphorylation
S256 Phosphorylation
K268 Sumoylation
T269 Phosphorylation
S295 Phosphorylation
K302 Ubiquitination
K315 Sumoylation
T326 Phosphorylation
S328 Phosphorylation
R336 Methylation
T341 Phosphorylation
S343 Phosphorylation
K359 Acetylation
T363 Phosphorylation
T364 Phosphorylation
S365 Phosphorylation
S376 Phosphorylation
S377 Phosphorylation
S384 Phosphorylation
S386 Phosphorylation
S388 Phosphorylation
T393 Phosphorylation
S400 Phosphorylation
T408 Phosphorylation
S410 Phosphorylation
T414 Phosphorylation
T420 Phosphorylation
S424 Phosphorylation
S425 Phosphorylation
S434 Phosphorylation
S453 Phosphorylation
T457 Phosphorylation
T461 Phosphorylation
S478 Phosphorylation
S481 Phosphorylation
S482 Phosphorylation
S484 Phosphorylation
T486 Phosphorylation
S490 Phosphorylation
S499 Phosphorylation
K506 Ubiquitination
Y512 Phosphorylation
S513 Phosphorylation
K516 Ubiquitination
S522 Phosphorylation
R524 Methylation
S525 Phosphorylation
K532 Sumoylation
K540 Ubiquitination
K548 Ubiquitination
S551 Phosphorylation
S561 Phosphorylation
T563 Phosphorylation
S583 Phosphorylation
S584 Phosphorylation
S585 Phosphorylation
S586 Phosphorylation
S588 Phosphorylation
S595 Phosphorylation
S599 Phosphorylation
S601 Phosphorylation
S605 Phosphorylation
S635 Phosphorylation
S637 Phosphorylation
S645 Phosphorylation
K654 Methylation
S655 Phosphorylation
S657 Phosphorylation
S661 Phosphorylation
S664 Phosphorylation
T666 Phosphorylation
Y668 Phosphorylation
T671 Phosphorylation
S675 Phosphorylation
S676 Phosphorylation
T682 Phosphorylation
C691 S-Nitrosylation
T697 Phosphorylation
S698 Phosphorylation
T699 Phosphorylation
S702 Phosphorylation
S703 Phosphorylation
S710 Phosphorylation
S714 Phosphorylation
K717 Acetylation
T720 Phosphorylation
S729 Phosphorylation
T744 Phosphorylation
T748 Phosphorylation
T751 Phosphorylation
S754 Phosphorylation
S814 Phosphorylation
S815 Phosphorylation
K818 Ubiquitination
S819 Phosphorylation
K823 Ubiquitination
S825 Phosphorylation
S828 Phosphorylation
T830 Phosphorylation
K831 Ubiquitination
S838 Phosphorylation
T840 Phosphorylation
K860 Ubiquitination
K861 Ubiquitination
S863 Phosphorylation
S865 Phosphorylation
S895 Phosphorylation
S898 Phosphorylation
T902 Phosphorylation
T976 Phosphorylation
T978 Phosphorylation
S981 Phosphorylation
S983 Phosphorylation
K986 Ubiquitination
S987 Phosphorylation
S990 Phosphorylation
T992 Phosphorylation
T999 Phosphorylation
S1004 Phosphorylation
K1029 Ubiquitination
K1047 Ubiquitination
S1104 Phosphorylation
T1106 Phosphorylation
S1161 Phosphorylation
S1176 Phosphorylation
S1178 Phosphorylation
S1180 Phosphorylation P78362 (SRPK2) , P31749 (AKT1)
K1209 Ubiquitination
K1219 Ubiquitination
Y1225 Phosphorylation
T1230 Phosphorylation
S1232 Phosphorylation
K1237 Ubiquitination
K1253 Methylation
S1284 Phosphorylation
S1325 Phosphorylation
S1327 Phosphorylation
S1329 Phosphorylation P31749 (AKT1)
S1331 Phosphorylation
T1332 Phosphorylation

研究背景

機能:

Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells.

PTMs:

Phosphorylation on Ser-1180 by SRPK2 up-regulates its stimulatory effect on cyclin A1.

Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.

細胞の位置付け:

Nucleus. Nucleus speckle. Nucleus>Nucleoplasm.
Note: Phosphorylation on Ser-1180 by SRPK2 redistributes it from the nuclear speckles to the nucleoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous. The Ser-1180 phosphorylated form (by SRPK2) is highly expressed and phosphorylated in patients with myeloid hematologic malignancies.

サブユニット構造:

Found in a mRNA splicing-dependent exon junction complex (EJC). Component of the heterotrimeric ASAP (apoptosis- and splicing-associated protein) complexes consisting of RNPS1, SAP18 and different isoforms of ACIN1; the association of SAP18 seems to require a preformed RNPS1:ACIN1 complex. Interacts with API5. Interacts with SRPK2 in a phosphorylation-dependent manner.

研究領域

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Genetic Information Processing > Transcription > Spliceosome.

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