Cytokeratin 18 Antibody - #AF0191

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製品: Cytokeratin 18 Antibody
カタログ: AF0191
タンパク質の説明: Rabbit polyclonal antibody to Cytokeratin 18
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC, IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Sheep, Rabbit
分子量: 46kDa; 48kD(Calculated).
ユニプロット: P05783
RRID: AB_2833384

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(88%), Sheep(82%), Rabbit(94%)
クローナリティ:
Polyclonal
特異性:
Cytokeratin 18 Antibody detects endogenous levels of total Cytokeratin 18.
RRID:
AB_2833384
引用形式: Affinity Biosciences Cat# AF0191, RRID:AB_2833384.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Cell proliferation inducing gene 46 protein; Cell proliferation inducing protein 46; Cell proliferation-inducing gene 46 protein; CK 18; CK-18; CK18; CYK 18; CYK18; Cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; K 18; K18; K1C18_HUMAN; KA18; Keratin 18; Keratin 18, type I; Keratin D; keratin, type I cytoskeletal 18; Keratin-18; Krt18;

免疫原

免疫原:

A synthesized peptide derived from human Cytokeratin 18, corresponding to a region within C-terminal amino acids.

Uniprot:

P05783(K1C18_HUMAN) >>Visit HPA database.

遺伝子(ID):
KRT18(3875)
発現特異性:
P05783 K1C18_HUMAN:

Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

タンパク質の説明:
K18 a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body.
タンパク質配列:
MSFTTRSTFSTNYRSLGSVQAPSYGARPVSSAASVYAGAGGSGSRISVSRSTSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRLASYLDRVRSLETENRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
94
Pig
88
Sheep
82
Zebrafish
64
Horse
0
Bovine
0
Dog
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

PTMs:

Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.

Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.

O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.

細胞の位置付け:

Cytoplasm>Perinuclear region. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

タンパク質ファミリー:

Belongs to the intermediate filament family.

研究領域

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

参考文献

1). LPS Disrupts Endometrial Receptivity by Inhibiting STAT1 Phosphorylation in Sheep. International journal of molecular sciences, 2024 (PubMed: 39769435) [IF=5.6]
2). Establishment and Characterization of a New Cell Line from Enzootic Nasal Adenocarcinoma in Goats: ENA-1. Veterinary sciences, 2024 (PubMed: 38922007) [IF=2.4]

Application: IF/ICC    Species: Mouse    Sample: ENA-1 cells

Figure 5 Immunofluorescence staining of ENA-1 cells for CK7 and CK18. ENA-1 cells expressed CK7 and CK18 in the cytoplasm, with CK18 most strongly expressed. (A) Negative control; (B) ENA-1 cells expressing CK7; and (C) ENA-1 cells strongly expressing CK18. (A–C), bar—100 μm.
3). Attenuation of renal fibrosis in mice due to lack of bombesin receptor-activated protein homologue. Clinical and experimental pharmacology & physiology, 2024 (PubMed: 39155151) [IF=2.4]
4). Bronchioloalveolar carcinoma not related to jaagsiekte sheep retrovirus in a goat. Veterinary pathology, 2007 (PubMed: 17846248) [IF=2.3]
5). Comparative study of the external auditory canal in humans and large mammals. The Anatomical Record, 2022 (PubMed: 34050614) [IF=1.8]

Application: IHC    Species: Human，Goat，Dog，Pig    Sample: EAC glandular cells

FIGURE 4 Immunostaining of cytoskeletal proteins of the ceruminous gland of a (a) human, (b) goat, (c) dog, and (d) pig. CK7, CK8, CK18, and CK19 are expressed in the EAC glandular cells of humans, goats, dogs, and pig. The positive expressions appeared to be brown or dark brown. Scale bar = 50 μm
6). TGFβ2 mediates oxidative stress-induced epithelial-to-mesenchymal transition of bladder smooth muscle. In vitro cellular & developmental biology. Animal, 2024 (PubMed: 38409639) [IF=1.5]

Application: WB    Species: Rat    Sample:

Figure 4. Expression of E-cadherin, CK-18, N-cadherin, vimentin, and α-SMA in bladder tissue of control and BOO rats. The mRNA expression levels of (a) E-cadherin, (b) CK-18, (c) N-cadherin, (d) vimentin, and (e) α-SMA were detected using RT-qPCR. ( f ) The protein expression levels of E-cadherin, N-cadherin, vimentin, CK-18, and α-SMA were detected using western blotting. The data are presented as the mean ± SD; **P 
7). Isolation and characterization of small extracellular vesicles from murine primary mammary tumor. STAR protocols, 2024 (PubMed: 38103192) [IF=1.3]

Application: WB    Species: Mouse    Sample: liver, adipose, and tumor tissue

Figure 3 Characterization of EVs isolated from liver and adipose tissue and tissue immune markers for EVs (A) Particle concentration (particles/mL) of isolated liver EVs acquired by nano-flow cytometry. Data are represented as mean ± SD of three independent biological replicates (n = 3). P value (P): ∗P < 0.05, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001, unpaired two-tailed Student’s t test. (B) Particle concentration (particles/mL) of isolated adipose tissue EVs acquired by nano-flow cytometry. Data are represented as mean ± SD of three independent biological replicates (n = 3). P value (P): ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001, unpaired two-tailed Student’s t test. (C) Transmission electron microscopy (TEM) images of EVs derived from liver. Scale bar, 100 nm. (D) Transmission electron microscopy (TEM) images of EVs derived from adipose tissue. Scale bar, 100 nm. (E) Immunoblotting analysis of samples from liver, adipose, and tumor tissue derived EVs.

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