HIPK1 Antibody - #DF8847
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8847, RRID:AB_2842044.
折りたたみ／展開
Hipk 1; Hipk1; HIPK1_HUMAN; Homeodomain interacting protein kinase 1; Homeodomain interacting protein kinase 1 like protein; Homeodomain-interacting protein kinase 1; Myak; Nbak 2; Nbak2; Nuclear body associated kinase 2; Nuclear body associated kinase 2b;
免疫原
Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines. Isoform 2 is highly expressed in testis. Expressed in both androgen-dependent and androgen-independent prostate cancer cells (PubMed:28289210).
- Q86Z02 HIPK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASQLQVFSPPSVSSSAFCSAKKLKIEPSGWDVSGQSSNDKYYTHSKTLPATQGQANSSHQVANFNIPAYDQGLLLPAPAVEHIVVTAADSSGSAATSTFQSSQTLTHRSNVSLLEPYQKCGLKRKSEEVDSNGSVQIIEEHPPLMLQNRTVVGAAATTTTVTTKSSSSSGEGDYQLVQHEILCSMTNSYEVLEFLGRGTFGQVAKCWKRSTKEIVAIKILKNHPSYARQGQIEVSILSRLSSENADEYNFVRSYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDPNLGYPLWRLKTPEEHELETGIKSKEARKYIFNCLDDMAQVNMSTDLEGTDMLAEKADRREYIDLLKKMLTIDADKRITPLKTLNHQFVTMTHLLDFPHSNHVKSCFQNMEICKRRVHMYDTVSQIKSPFTTHVAPNTSTNLTMSFSNQLNTVHNQASVLASSSTAAAATLSLANSDVSLLNYQSALYPSSAAPVPGVAQQGVSLQPGTTQICTQTDPFQQTFIVCPPAFQTGLQATTKHSGFPVRMDNAVPIVPQAPAAQPLQIQSGVLTQGSCTPLMVATLHPQVATITPQYAVPFTLSCAAGRPALVEQTAAVLQAWPGGTQQILLPSTWQQLPGVALHNSVQPTAMIPEAMGSGQQLADWRNAHSHGNQYSTIMQQPSLLTNHVTLATAQPLNVGVAHVVRQQQSSSLPSKKNKQSAPVSSKSSLDVLPSQVYSLVGSSPLRTTSSYNSLVPVQDQHQPIIIPDTPSPPVSVITIRSDTDEEEDNKYKPSSSGLKPRSNVISYVTVNDSPDSDSSLSSPYSTDTLSALRGNSGSVLEGPGRVVADGTGTRTIIVPPLKTQLGDCTVATQASGLLSNKTKPVASVSGQSSGCCITPTGYRAQRGGTSAAQPLNLSQNQQSSAAPTSQERSSNPAPRRQQAFVAPLSQAPYTFQHGSPLHSTGHPHLAPAPAHLPSQAHLYTYAAPTSAAALGSTSSIAHLFSPQGSSRHAAAYTTHPSTLVHQVPVSVGPSLLTSASVAPAQYQHQFATQSYIGSSRGSTIYTGYPLSPTKISQYSYL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q86Z02 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S9 | Phosphorylation | Uniprot | |
| K25 | Sumoylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| K120 | Sumoylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| T351 | Phosphorylation | Uniprot | |
| Y352 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| K497 | Ubiquitination | Uniprot | |
| K505 | Ubiquitination | Uniprot | |
| K543 | Ubiquitination | Uniprot | |
| S854 | Phosphorylation | Uniprot | |
| K855 | Ubiquitination | Uniprot | |
| S872 | Phosphorylation | Uniprot | |
| T876 | Phosphorylation | Uniprot | |
| S879 | Phosphorylation | Uniprot | |
| K921 | Ubiquitination | Uniprot | |
| K928 | Ubiquitination | Uniprot | |
| S965 | Phosphorylation | Uniprot | |
| S967 | Phosphorylation | Uniprot | |
| K991 | Ubiquitination | Uniprot | |
| T1027 | Phosphorylation | Uniprot | |
| R1035 | Methylation | Uniprot | |
| S1063 | Phosphorylation | Uniprot | |
| Y1197 | Phosphorylation | Uniprot | |
| S1200 | Phosphorylation | Uniprot | |
| T1202 | Phosphorylation | Uniprot |
研究背景
Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation. Phosphorylates PAGE4 at 'Thr-51' which is critical for the ability of PAGE4 to potentiate the transcriptional activator activity of JUN.
Autophosphorylated. Phosphorylated and activated by JNK1.
Degraded by PARK7 at the protein level.
Sumoylated. When conjugated it is directed to nuclear speckles. SENP1-mediated desumoylation is mediated by TNF in response to stress stimuli, triggering transient translocation from nucleus to cytoplasm.
Nucleus. Cytoplasm. Nucleus speckle.
Note: Predominantly nuclear. Translocates from nucleus to cytoplasm in response to stress stimuli via SENP1-mediated desumoylation.
Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines. Isoform 2 is highly expressed in testis. Expressed in both androgen-dependent and androgen-independent prostate cancer cells.
Interacts with Nkx1-2, Nkx2-5, MYB, PARK7, DAXX and p53/TP53. Part of a cytoplasmic complex made of HIPK1, DAB2IP and MAP3K5 in response to TNF. This complex formation promotes MAP3K5-JNK activation and subsequent apoptosis.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
研究領域
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.