Cytokeratin 16 Antibody - #AF0189

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製品: Cytokeratin 16 Antibody
カタログ: AF0189
タンパク質の説明: Rabbit polyclonal antibody to Cytokeratin 16
アプリケーション: WB IHC IF/ICC
Cited expt.: IHC
反応性: Human, Mouse, Rat
予測: Rabbit
分子量: 50~60kD; 51kD(Calculated).
ユニプロット: P08779
RRID: AB_2833382

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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
Cytokeratin 16 Antibody detects endogenous levels of total Cytokeratin 16.
RRID:
AB_2833382
引用形式: Affinity Biosciences Cat# AF0189, RRID:AB_2833382.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CK 16; CK-16; CK16; Cytokeratin-16; Cytokeratin16; FNEPPK; Focal non epidermolytic palmoplantar keratoderma; K 16; K16; K1C16_HUMAN; K1CP; Keratin 1 type I; Keratin 16; Keratin; Keratin type I cytoskeletal 16; Keratin-16; Keratin16; KRT 16; Krt16; KRT16A; NEPPK; PC1; type I cytoskeletal 16;

免疫原

免疫原:

A synthesized peptide derived from human Cytokeratin 16, corresponding to a region within C-terminal amino acids.

Uniprot:

P08779(K1C16_HUMAN) >>Visit HPA database.

遺伝子(ID):
KRT16(3868)
発現特異性:
P08779 K1C16_HUMAN:

Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.

タンパク質の説明:
K16 a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K6. K16 and K17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.
タンパク質配列:
MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSRFSSGGACGLGGGYGGGFSSSSSFGSGFGGGYGGGLGAGFGGGLGAGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPSEIKDYSPYFKTIEDLRNKIIAATIENAQPILQIDNARLAADDFRTKYEHELALRQTVEADVNGLRRVLDELTLARTDLEMQIEGLKEELAYLRKNHEEEMLALRGQTGGDVNVEMDAAPGVDLSRILNEMRDQYEQMAEKNRRDAETWFLSKTEELNKEVASNSELVQSSRSEVTELRRVLQGLEIELQSQLSMKASLENSLEETKGRYCMQLSQIQGLIGSVEEQLAQLRCEMEQQSQEYQILLDVKTRLEQEIATYRRLLEGEDAHLSSQQASGQSYSSREVFTSSSSSSSRQTRPILKEQSSSSFSQGQSS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.

組織特異性:

Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.

タンパク質ファミリー:

Belongs to the intermediate filament family.

研究領域

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

参考文献

1). LINC00853 contributes to tumor stemness of gastric cancer through FOXP3-mediated transcription of PDZK1IP1. Biological procedures online, 2023 (PubMed: 37403034) [IF=6.4]

Application: IHC    Species: human    Sample:

Fig. 6 From: LINC00853 contributes to tumor stemness of gastric cancer through FOXP3-mediated transcription of PDZK1IP1 LINC00853 significantly accelerated tumor progression of GC cells in vivo. The photos of the tumor xenografts (A) and the tumor volume curve (B-C) and the tumor weight graph (D) of xenograft tumors generated via subcutaneous administration of MGC803 and MKN-78 cells stably transfected with sh-LINC00853 or LINC00853 OE, respectively. E Detection of proliferation in tumor tissues with Ki67 staining assay. Scale bar = 50 μm. F ImageJ software was utilized to quantify the number of Ki67-positive cells in tumor tissue. G The level of LINC00853 was clarified via RT-PCR test. H, I Western blot assay was applied to illustrate the level of PDZK1IP1. *p 

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