FOXI1 Antibody - #DF8818

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製品: FOXI1 Antibody
カタログ: DF8818
タンパク質の説明: Rabbit polyclonal antibody to FOXI1
アプリケーション: WB
Cited expt.: WB
反応性: Human, Mouse
予測: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 40 kDa; 41kD(Calculated).
ユニプロット: Q12951
RRID: AB_2842015

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(80%), Dog(80%)
クローナリティ:
Polyclonal
特異性:
FOXI1 Antibody detects endogenous levels of total FOXI1.
RRID:
AB_2842015
引用形式: Affinity Biosciences Cat# DF8818, RRID:AB_2842015.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FKH10; FKHL10; Forkhead (Drosophila) like 10; Forkhead box I1; Forkhead box protein I1; Forkhead like 10; Forkhead related activator 6; Forkhead related transcription factor 6; Forkhead-related protein FKHL10; Forkhead-related transcription factor 6; FOXI1; FOXI1_HUMAN; FREAC 6; FREAC-6; FREAC6; Hepatocyte nuclear factor 3 forkhead homolog 3; HFH 3; HFH-3; HFH3; HNF 3/fork head homolog 3; HNF-3 fork-head homolog 3; HNF-3/fork-head homolog 3; Human HNF-3 fork-head homolog-3 HFH-3 mRNA complete cds; MGC34197;

免疫原

免疫原:

A synthesized peptide derived from human FOXI1, corresponding to a region within C-terminal amino acids.

Uniprot:

Q12951(FOXI1_HUMAN) >>Visit HPA database.

遺伝子(ID):
FOXI1(2299)
発現特異性:
Q12951 FOXI1_HUMAN:

Expressed in kidney.

タンパク質配列:
MSSFDLPAPSPPRCSPQFPSIGQEPPEMNLYYENFFHPQGVPSPQRPSFEGGGEYGATPNPYLWFNGPTMTPPPYLPGPNASPFLPQAYGVQRPLLPSVSGLGGSDLGWLPIPSQEELMKLVRPPYSYSALIAMAIHGAPDKRLTLSQIYQYVADNFPFYNKSKAGWQNSIRHNLSLNDCFKKVPRDEDDPGKGNYWTLDPNCEKMFDNGNFRRKRKRKSDVSSSTASLALEKTESSLPVDSPKTTEPQDILDGASPGGTTSSPEKRPSPPPSGAPCLNSFLSSMTAYVSGGSPTSHPLVTPGLSPEPSDKTGQNSLTFNSFSPLTNLSNHSGGGDWANPMPTNMLSYGGSVLSQFSPHFYNSVNTSGVLYPREGTEV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
80
Rabbit
80
Pig
70
Xenopus
60
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Transcriptional activator required for the development of normal hearing, sense of balance and kidney function. Required for the expression of SLC26A4/PDS, JAG1 and COCH in a subset of epithelial cells and the development of the endolymphatic system in the inner ear. Also required for the expression of SLC4A1/AE1, SLC4A9/AE4, ATP6V1B1 and the differentiation of intercalated cells in the epithelium of distal renal tubules (By similarity).

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in kidney.

参考文献

1). Transcriptomics reveals age-related changes in ion transport-related factors in yak lungs. Frontiers in veterinary science, 2024 (PubMed: 38779034) [IF=3.2]

Application: WB    Species: yaks    Sample: lungs

Figure 6. FOXI1, KCNMA1, and SLC12A2 mRNA and protein expression in lungs of yaks of different ages. (A) FOXI1, KCNMA1, and SLC12A2 mRNA expression results. (B) Western blots for FOXI1, KCNMA1, and SLC12A2. (C) Western blotting results of FOXI1, KCNMA1, and SLC12A2 expression. 1, 2, and 3 denote newborn, juvenile, and adult yaks, respectively. *significant (p  0.05).

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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