ADCK4 Antibody - #DF8634

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製品: ADCK4 Antibody
カタログ: DF8634
タンパク質の説明: Rabbit polyclonal antibody to ADCK4
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 60 kDa; 60kD(Calculated).
ユニプロット: Q96D53
RRID: AB_2841838

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 100ul $280 在庫あり
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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
ADCK4 Antibody detects endogenous levels of total ADCK4.
RRID:
AB_2841838
引用形式: Affinity Biosciences Cat# DF8634, RRID:AB_2841838.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

aarF domain containing protein kinase 4; ADCK4; ADCK4_HUMAN; NPHS9; Uncharacterized aarF domain containing protein kinase 4; Uncharacterized aarF domain-containing protein kinase 4;

免疫原

免疫原:

A synthesized peptide derived from human ADCK4, corresponding to a region within N-terminal amino acids.

Uniprot:

Q96D53(COQ8B_HUMAN) >>Visit HPA database.

遺伝子(ID):
COQ8B(79934)
発現特異性:
Q96D53 COQ8B_HUMAN:

Widely expressed, including renal podocytes.

タンパク質配列:
MWLKVGGLLRGTGGQLGQTVGWPCGALGPGPHRWGPCGGSWAQKFYQDGPGRGLGEEDIRRAREARPRKTPRPQLSDRSRERKVPASRISRLANFGGLAVGLGLGVLAEMAKKSMPGGRLQSEGGSGLDSSPFLSEANAERIVQTLCTVRGAALKVGQMLSIQDNSFISPQLQHIFERVRQSADFMPRWQMLRVLEEELGRDWQAKVASLEEVPFAAASIGQVHQGLLRDGTEVAVKIQYPGIAQSIQSDVQNLLAVLKMSAALPAGLFAEQSLQALQQELAWECDYRREAACAQNFRQLLANDPFFRVPAVVKELCTTRVLGMELAGGVPLDQCQGLSQDLRNQICFQLLTLCLRELFEFRFMQTDPNWANFLYDASSHQVTLLDFGASREFGTEFTDHYIEVVKAAADGDRDCVLQKSRDLKFLTGFETKAFSDAHVEAVMILGEPFATQGPYDFGSGETARRIQDLIPVLLRHRLCPPPEETYALHRKLAGAFLACAHLRAHIACRDLFQDTYHRYWASRQPDAATAGSLPTKGDSWVDPS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
92
Xenopus
92
Chicken
82
Zebrafish
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration. Its substrate specificity is unclear: does not show any protein kinase activity. Probably acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway. Required for podocyte migration.

細胞の位置付け:

Mitochondrion membrane>Single-pass membrane protein. Cytoplasm>Cytosol. Cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed, including renal podocytes.

タンパク質ファミリー:

Adopts an atypical protein kinase-like fold: while it adopts a core fold similar to that of well-characterized protein kinase-like domains. The KxGQ motif completely occludes the typical substrate binding pocket. Nucleotide-binding opens the substrate binding pocket and flips the active site from inside the hydrophobic core into a catalytically competent, solvent-exposed posture.

Belongs to the protein kinase superfamily. ADCK protein kinase family.

参考文献

1). Voluntary wheel exercise improves glymphatic clearance and ameliorates colitis-associated cognitive impairment in aged mice by inhibiting TRPV4-induced astrocytic calcium activity. Experimental neurology, 2024 (PubMed: 38580155) [IF=4.6]
2). Transcription factor Kruppel-like factor 5 positively regulates the expression of AarF domain containing kinase 4. MOLECULAR BIOLOGY REPORTS, 2020 (PubMed: 33033902) [IF=2.6]

Application: WB    Species: human    Sample: HEK293

Fig. 4| KLF5 positively regulated the expression of ADCK4. a, c, eThe expression of KLF5 increased and decreased after transfection with pENTER-KLF5 and KLF5 siRNA. b, d, e After overexpression or knockdown of KLF5, the mRNA and protein expression levels of ADCK4 separately increased or decreased.

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