Synphilin-1 Antibody - #DF8619

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製品: Synphilin-1 Antibody
カタログ: DF8619
タンパク質の説明: Rabbit polyclonal antibody to Synphilin-1
アプリケーション: WB IHC IF/ICC
Cited expt.: IF/ICC
反応性: Human, Mouse
予測: Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 100 kDa; 100kD(Calculated).
ユニプロット: Q9Y6H5
RRID: AB_2841823

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説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(88%), Xenopus(88%)
クローナリティ:
Polyclonal
特異性:
Synphilin-1 Antibody detects endogenous levels of total Synphilin-1.
RRID:
AB_2841823
引用形式: Affinity Biosciences Cat# DF8619, RRID:AB_2841823.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Alpha synuclein interacting protein; Alpha-synuclein-interacting protein; MGC39814; SNCAIP; SNCAP_HUMAN; Sph1; Synphilin-1; Synphilin1; Synuclein alpha interacting protein (synphilin); Synuclein alpha interacting protein; SYPH 1;

免疫原

免疫原:

A synthesized peptide derived from human Synphilin-1, corresponding to a region within the internal amino acids.

Uniprot:

Q9Y6H5(SNCAP_HUMAN) >>Visit HPA database.

遺伝子(ID):
SNCAIP(9627)
発現特異性:
Q9Y6H5 SNCAP_HUMAN:

Detected in brain (at protein level). Widely expressed, with highest levels in brain, heart and placenta.

タンパク質配列:
MEAPEYLDLDEIDFSDDISYSVTSLKTIPELCRRCDTQNEDRSVSSSSWNCGISTLITNTQKPTGIADVYSKFRPVKRVSPLKHQPETLENNESDDQKNQKVVEYQKGGESDLGPQPQELGPGDGVGGPPGKSSEPSTSLGELEHYDLDMDEILDVPYIKSSQQLASFTKVTSEKRILGLCTTINGLSGKACSTGSSESSSSNMAPFCVLSPVKSPHLRKASAVIHDQHKLSTEETEISPPLVKCGSAYEPENQSKDFLNKTFSDPHGRKVEKTTPDCQLRAFHLQSSAAESKPEEQVSGLNRTSSQGPEERSEYLKKVKSILNIVKEGQISLLPHLAADNLDKIHDENGNNLLHIAASQGHAECLQHLTSLMGEDCLNERNTEKLTPAGLAIKNGQLECVRWMVSETEAIAELSCSKDFPSLIHYAGCYGQEKILLWLLQFMQEQGISLDEVDQDGNSAVHVASQHGYLGCIQTLVEYGANVTMQNHAGEKPSQSAERQGHTLCSRYLVVVETCMSLASQVVKLTKQLKEQTVERVTLQNQLQQFLEAQKSEGKSLPSSPSSPSSPASRKSQWKSPDADDDSVAKSKPGVQEGIQVLGSLSASSRARPKAKDEDSDKILRQLLGKEISENVCTQEKLSLEFQDAQASSRNSKKIPLEKRELKLARLRQLMQRSLSESDTDSNNSEDPKTTPVRKADRPRPQPIVESVESMDSAESLHLMIKKHTLASGGRRFPFSIKASKSLDGHSPSPTSESSEPDLESQYPGSGSIPPNQPSGDPQQPSPDSTAAQKVATSPKSALKSPSSKRRTSQNLKLRVTFEEPVVQMEQPSLELNGEKDKDKGRTLQRTSTSNESGDQLKRPFGAFRSIMETLSGNQNNNNNYQAANQLKTSTLPLTSLGRKTDAKGNPASSASKGKNKAA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
88
Chicken
88
Pig
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1.

PTMs:

Ubiquitinated; mediated by SIAH1, SIAH2 or RNF19A and leading to its subsequent proteasomal degradation. In the absence of proteasomal degradation, ubiquitinated SNCAIP accumulates in cytoplasmic inclusion bodies. Isoform 2 is subject to limited ubiquitination that does not lead to proteasomal degradation.

細胞の位置付け:

Cytoplasm.
Note: Detected in cytoplasmic inclusion bodies, together with SNCA.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in brain (at protein level). Widely expressed, with highest levels in brain, heart and placenta.

研究領域

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

参考文献

1). Maturation and detoxification of synphilin-1 inclusion bodies regulated by sphingolipids. eLife, 2025 (PubMed: 39927758) [IF=6.4]

Application: IF/ICC    Species: human    Sample: HEK293T cell

Figure 7 Sphingolipids regulate SY1-induced cytotoxicity and SY1 IB morphology in mammalian cells. (A) Inhibition of the intracellular sphingolipid synthesis pathway by myriocin enhanced SY1 cytotoxicity based on mitochondrial membrane potential and ATP content assays. Data were analyzed using the Student’s t-test. Error bars indicate the SD, n=5-9 biological replicates. (B) Addition of exogenous DHS rescued the cytotoxicity caused by SY1 expression. Data were analyzed using the Student’s t-test. Error bars indicate the SD, n=4-9 biological replicates. (C) Exogenous addition of DHS to SPTLC2 knockout cells restored the typical spherical SY1 IBs in HEK293t. Statistics of the ratio of cells with dispersed irregular IBs after exogenous addition of DMSO and DHS to HEK293t SPTLC2 knockout cells (See also Figure 7—figure supplement 2). Scale bar = 20 μm. Data were analyzed using the Student’s t-test. Error bars indicate the SD, n=5 biological replicates. (D) DHS ameliorated SY1 cytotoxicity by normalizing mitochondrial membrane potential and cell viability. Data were analyzed using the Student’s t-test. Error bars indicate the SD, n=6 biological replicates.

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