SP-B Antibody - #DF8615

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製品: SP-B Antibody
カタログ: DF8615
タンパク質の説明: Rabbit polyclonal antibody to SP-B
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human
分子量: 42 kDa; 42kD(Calculated).
ユニプロット: P07988
RRID: AB_2841819

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
SP-B Antibody detects endogenous levels of total SP-B.
RRID:
AB_2841819
引用形式: Affinity Biosciences Cat# DF8615, RRID:AB_2841819.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

18 kDa pulmonary-surfactant protein; 6 kDa protein; PSP B; PSPB; PSPB_HUMAN; Pulmonary surfactant apoprotein PSP-B; Pulmonary surfactant-associated protein B; Pulmonary surfactant-associated protein, 18-KD; Pulmonary surfactant-associated proteolipid SPL(Phe); SFTB3; SFTP3; SFTPB; SMDP1; SP B; SP-B; SPB; surfactant protein B; Surfactant, pulmonary-associated protein B; surfactant-associated protein, pulmonary, 3;

免疫原

免疫原:

A synthesized peptide derived from human SP-B, corresponding to a region within the internal amino acids.

Uniprot:

P07988(PSPB_HUMAN) >>Visit HPA database.

遺伝子(ID):
SFTPB(6439)
タンパク質配列:
MAESHLLQWLLLLLPTLCGPGTAAWTTSSLACAQGPEFWCQSLEQALQCRALGHCLQEVWGHVGADDLCQECEDIVHILNKMAKEAIFQDTMRKFLEQECNVLPLKLLMPQCNQVLDDYFPLVIDYFQNQTDSNGICMHLGLCKSRQPEPEQEPGMSDPLPKPLRDPLPDPLLDKLVLPVLPGALQARPGPHTQDLSEQQFPIPLPYCWLCRALIKRIQAMIPKGALAVAVAQVCRVVPLVAGGICQCLAERYSVILLDTLLGRMLPQLVCRLVLRCSMDDSAGPRSPTGEWLPRDSECHLCMSVTTQAGNSSEQAIPQAMLQACVGSWLDREKCKQFVEQHTPQLLTLVPRGWDAHTTCQALGVCGTMSSPLQCIHSPDL

研究背景

機能:

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

細胞の位置付け:

Secreted>Extracellular space>Surface film.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

参考文献

1). miR-296-5p Inhibits the Secretion of Pulmonary Surfactants in Pulmonary Epithelial Cells via the Downregulation of Wnt7b/β-Catenin Signaling. Biomed Research International, 2021 (PubMed: 33490270) [IF=2.6]

Application: WB    Species: Human    Sample: A549 cell

Figure 4 miR-296-5p inhibited the expression of SP-A (SFTPA1) and SP-B through the Wnt signaling pathway. After transfection of mir-296-5p overexpression and empty vectors in the A549 cell line, the expression of WNT7B, β-catenin, SP-A, and SP-B was assessed using western blotting.

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