RNase III Drosha Antibody - #DF8601
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| 製品: | RNase III Drosha Antibody |
| カタログ: | DF8601 |
| タンパク質の説明: | Rabbit polyclonal antibody to RNase III Drosha |
| アプリケーション: | WB |
| 反応性: | Human, Mouse |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken |
| 分子量: | 159 kDa; 159kD(Calculated). |
| ユニプロット: | Q9NRR4 |
| RRID: | AB_2841805 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8601, RRID:AB_2841805.
折りたたみ／展開
DROSHA; Drosha double stranded RNA specific endoribonuclease; Drosha ribonuclease type III; Etohi2; HSA242976; Nuclear RNase III Drosha; p241; Protein Drosha; Putative protein p241 which interacts with transcription factor Sp1; Putative ribonuclease III; RANSE3L; Ribonuclease 3; Ribonuclease III; Ribonuclease III nuclear; Ribonuclease type III nuclear; RibonucleaseIII; RN 3; RN3; RNase 3; RNase III; RNase3; RNASE3L; RNaseIII; RNASEN; RNC_HUMAN;
免疫原
- Q9NRR4 RNC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMQGNTCHRMSFHPGRGCPRGRGGHGARPSAPSFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRPPFPNHQMRHPFPVPPCFPPMPPPMPCPNNPPVPGAPPGQGTFPFMMPPPSMPHPPPPPVMPQQVNYQYPPGYSHHNFPPPSFNSFQNNPSSFLPSANNSSSPHFRHLPPYPLPKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLDRRERGRSPDRRRQDSRYRSDYDRGRTPSRHRSYERSRERERERHRHRDNRRSPSLERSYKKEYKRSGRSYGLSVVPEPAGCTPELPGEIIKNTDSWAPPLEIVNHRSPSREKKRARWEEEKDRWSDNQSSGKDKNYTSIKEKEPEETMPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGTKPSSVRIDQLDREQFNPDVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEEAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRWEREHQEREPDETEDIKK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NRR4 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S221 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| Y228 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| S267 | Phosphorylation | Uniprot | |
| Y269 | Phosphorylation | Uniprot | |
| R271 | Methylation | Uniprot | |
| T274 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| S276 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Q16539 (MAPK14) , P49841 (GSK3B) | Uniprot |
| S302 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S317 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| S357 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| K388 | Sumoylation | Uniprot | |
| K409 | Sumoylation | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| S491 | Phosphorylation | Uniprot | |
| S698 | Phosphorylation | Uniprot | |
| Y705 | Phosphorylation | Uniprot | |
| K738 | Ubiquitination | Uniprot | |
| T747 | Phosphorylation | Uniprot | |
| K748 | Ubiquitination | Uniprot | |
| K792 | Ubiquitination | Uniprot | |
| K795 | Ubiquitination | Uniprot | |
| S796 | Phosphorylation | Uniprot | |
| S807 | Phosphorylation | Uniprot | |
| K809 | Ubiquitination | Uniprot | |
| K827 | Ubiquitination | Uniprot | |
| K831 | Ubiquitination | Uniprot | |
| K1207 | Ubiquitination | Uniprot | |
| K1250 | Ubiquitination | Uniprot | |
| K1262 | Ubiquitination | Uniprot | |
| K1277 | Ubiquitination | Uniprot | |
| K1285 | Ubiquitination | Uniprot | |
| S1293 | Phosphorylation | Uniprot | |
| K1313 | Ubiquitination | Uniprot | |
| K1331 | Ubiquitination | Uniprot | |
| K1341 | Ubiquitination | Uniprot | |
| K1353 | Ubiquitination | Uniprot | |
| K1373 | Ubiquitination | Uniprot |
研究背景
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
Nucleus. Nucleus>Nucleolus.
Note: A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.
Ubiquitous.
Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DROSHA and DGCR8. The microprocessor complex is a heterotrimer; each of the two DROSHA RNase III domains binds one DGCR8 (via C-terminal region). Interacts with SP1 and SNIP1. Interacts with SRRT/ARS2 (By similarity). Interacts with CPSF3 and ISY1; this interaction is in an RNA dependent manner (By similarity).
The 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other.
Belongs to the ribonuclease III family.
研究領域
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
参考文献
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