OBCAM Antibody - #DF8584

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製品: OBCAM Antibody
カタログ: DF8584
タンパク質の説明: Rabbit polyclonal antibody to OBCAM
アプリケーション: WB
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 38 kDa; 38kD(Calculated).
ユニプロット: Q14982
RRID: AB_2841788

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 100ul $280 在庫あり
 200ul $350 在庫あり

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(92%)
クローナリティ:
Polyclonal
特異性:
OBCAM Antibody detects endogenous levels of total OBCAM.
RRID:
AB_2841788
引用形式: Affinity Biosciences Cat# DF8584, RRID:AB_2841788.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

GM181; IgLON family member 1; IGLON1; OBCAM; OPCM; OPCM_HUMAN; OPCML; Opiate binding cell adhesion molecule; Opioid binding cell adhesion molecule; Opioid binding protein/cell adhesion molecule; Opioid binding protein/cell adhesion molecule-like; Opioid binding protein/cell adhesion molecule-like preprotein; Opioid-binding cell adhesion molecule; Opioid-binding protein/cell adhesion molecule;

免疫原

免疫原:

A synthesized peptide derived from human OBCAM, corresponding to a region within C-terminal amino acids.

Uniprot:

Q14982(OPCM_HUMAN) >>Visit HPA database.

遺伝子(ID):
OPCML(4978)
タンパク質配列:
MGVCGYLFLPWKCLVVVSLRLLFLVPTGVPVRSGDATFPKAMDNVTVRQGESATLRCTIDDRVTRVAWLNRSTILYAGNDKWSIDPRVIILVNTPTQYSIMIQNVDVYDEGPYTCSVQTDNHPKTSRVHLIVQVPPQIMNISSDITVNEGSSVTLLCLAIGRPEPTVTWRHLSVKEGQGFVSEDEYLEISDIKRDQSGEYECSALNDVAAPDVRKVKITVNYPPYISKAKNTGVSVGQKGILSCEASAVPMAEFQWFKEETRLATGLDGMRIENKGRMSTLTFFNVSEKDYGNYTCVATNKLGNTNASITLYGPGAVIDGVNSASRALACLWLSGTLLAHFFIKF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
92
Bovine
92
Sheep
92
Chicken
92
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Binds opioids in the presence of acidic lipids; probably involved in cell contact.

細胞の位置付け:

Cell membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the immunoglobulin superfamily. IgLON family.

参考文献

1). Comprehensive upstream and downstream regulatory analyses identify miR-675-3p as a potential prognostic biomarker in melanoma. Human Cell, 2021 (PubMed: 33400243) [IF=3.4]

Application: WB    Species: Human    Sample: melanoma A375 cells

Fig. 5 Identifcation of miR-675-3p target genes. a Pearson’s correlation analyses showed that miR-675-3p was signifcantly positively correlated with ERG1 and negatively correlated with ZBTB7 and IGF1R expression in the TCGA database. b Validation of transfection efciency. c Relative target gene mRNA expression. d Relative target gene protein expression. e OPCML was targeted by miR-675-3p in a dual luciferase reporter experiment

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