MIP-1 alpha Antibody - #DF8572

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製品: MIP-1 alpha Antibody
カタログ: DF8572
タンパク質の説明: Rabbit polyclonal antibody to MIP-1 alpha
アプリケーション: WB IHC
Cited expt.: WB, IHC
反応性: Human
予測: Bovine, Horse
分子量: 10 kDa; 10kD(Calculated).
ユニプロット: P10147
RRID: AB_2841776

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Bovine(100%), Horse(86%)
クローナリティ:
Polyclonal
特異性:
MIP-1 alpha Antibody detects endogenous levels of total MIP-1 alpha.
RRID:
AB_2841776
引用形式: Affinity Biosciences Cat# DF8572, RRID:AB_2841776.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C C motif chemokine 3; CCL 3; CCL3; CCL3_HUMAN; Chemokine C C motif ligand 3; G0/G1 switch regulatory protein 19 1; G0/G1 switch regulatory protein 19-1; G0S19 1; G0S19 1 protein; Heparin binding chemotaxis protein; L2G25B; LD78 alpha; LD78-alpha(4-69); LD78alpha; Macrophage inflammatory protein 1 alpha; Macrophage inflammatory protein 1-alpha; MIP 1 alpha; MIP 1A; MIP-1-alpha; MIP-1-alpha(4-69); MIP1A; PAT 464.1; SCYA 3; SCYA3; SIS alpha; SIS beta; SIS-beta; Small inducible cytokine A3; small inducible cytokine A3 (homologous to mouse Mip-1a); Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein;

免疫原

免疫原:

A synthesized peptide derived from human MIP-1 alpha, corresponding to a region within the internal amino acids.

Uniprot:

P10147(CCL3_HUMAN) >>Visit HPA database.

遺伝子(ID):
CCL3(6348)
タンパク質配列:
MQVSTAALAVLLCTMALCNQFSASLAADTPTACCFSYTSRQIPQNFIADYFETSSQCSKPGVIFLTKRSRQVCADPSEEWVQKYVSDLELSA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Horse
86
Pig
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

PTMs:

N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the intercrine beta (chemokine CC) family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

参考文献

1). Identification of gene signatures associated with ulcerative colitis and the association with immune infiltrates in colon cancer. Frontiers in immunology, 2023 (PubMed: 36742294) [IF=5.7]

Application: IHC    Species: human    Sample:

Figure 5 Expression of CCL3, MMP3, and TIMP1 in UC patients and induced enteritis cells. (A) The expression of CCL3. (B) The expression of MMP3. (C) The expression of TIMP1. (D) The protein expression levels of CCL3, MMP3 and TIMP1 in UC patients and healthy controls. (E) The inflammatory factors IL-8 and IL-1β secretion level after 1 ug LPS stimulated NCM460 cells for 0h, 12h and 24h. (F) The protein expression levels of CCL3, MMP3 and TIMP1 in NCM460 cells stimulated with LPS for 0h, 12h and 24h.

Application: WB    Species: human    Sample:

Figure 5 Expression of CCL3, MMP3, and TIMP1 in UC patients and induced enteritis cells. (A) The expression of CCL3. (B) The expression of MMP3. (C) The expression of TIMP1. (D) The protein expression levels of CCL3, MMP3 and TIMP1 in UC patients and healthy controls. (E) The inflammatory factors IL-8 and IL-1β secretion level after 1 ug LPS stimulated NCM460 cells for 0h, 12h and 24h. (F) The protein expression levels of CCL3, MMP3 and TIMP1 in NCM460 cells stimulated with LPS for 0h, 12h and 24h.
2). Isosteviol reduces the acute inflammatory response after burns by upregulating MMP9 in macrophages leading to M2 polarization. International Immunopharmacology, 2022 (PubMed: 35176589) [IF=4.8]
3). Ghrelin inhibits early brain injury due to subarachnoid hemorrhage via the Tim-3-mediated HMGB1/NF-κB pathway. JOURNAL OF CHEMICAL NEUROANATOMY, 2022 (PubMed: 35863561) [IF=2.7]
4). Identification and preliminary validation of differently expressed genes as candidate biomarkers associated with atherosclerosis. Gene, 2024 (PubMed: 38527674) [IF=2.6]

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