Defensin beta-1 Antibody - #DF8530

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製品: Defensin beta-1 Antibody
カタログ: DF8530
タンパク質の説明: Rabbit polyclonal antibody to Defensin beta-1
アプリケーション: WB
Cited expt.: WB
反応性: Human
分子量: 7 kDa(Observed); 7kD(Calculated).
ユニプロット: P60022
RRID: AB_2841734

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MS Report
HPLC Report
MSDS
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
Defensin beta-1 Antibody detects endogenous levels of total Defensin beta-1.
RRID:
AB_2841734
引用形式: Affinity Biosciences Cat# DF8530, RRID:AB_2841734.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

BD 1; BD-1; BD1; beta 1; Beta defensin 1; Beta-defensin 1; DEFB 1; DEFB1; DEFB1_HUMAN; DEFB101; Defensin; Defensin beta 1; Defensin beta 1 preproprotein; HBD 1; hBD-1; HBD1; MGC51822;

免疫原

免疫原:

A synthesized peptide derived from human Defensin beta-1, corresponding to a region within the internal amino acids.

Uniprot:

P60022(DEFB1_HUMAN) >>Visit HPA database.

遺伝子(ID):
DEFB1(1672)
発現特異性:
P60022 DEFB1_HUMAN:

Blood plasma. Sperm. Highly expressed in the lower head and midpiece of sperm. Significantly reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level).

タンパク質配列:
MRTSYLLLFTLCLLLSEMASGGNFLTGLGHRSDHYNCVSSGGQCLYSACPIFTKIQGTCYRGKAKCCK

研究背景

機能:

Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility.

細胞の位置付け:

Secreted. Membrane.
Note: Associates with tumor cell membrane-derived microvesicles (PubMed:23938203).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Blood plasma. Sperm. Highly expressed in the lower head and midpiece of sperm. Significantly reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level).

タンパク質ファミリー:

Belongs to the beta-defensin family.

参考文献

1). Gut microbiota and butyrate contribute to nonalcoholic fatty liver disease in premenopause due to estrogen deficiency. PLoS One, 2022 (PubMed: 35108315) [IF=2.9]

Application: WB    Species: Mouse    Sample:

Fig 6. Decreased production of Reg3γ and β-defensins in IEC of OH mice. IEC were isolated from the small intestines of C, SH and OH mice, respectively. A: IEC expression of Reg3γ and β-defensins 1 and 3 in OH and the control mice were determined by qRT-PCR. B: Protein levels of Reg3γ and β-defensins 1 were determined by western blot. C: Relative density ratio of Reg3γ and β-defensins 1. IEC, intestinal epithelial cell; qRT-PCR, quantitative real-time polymerase chain reaction; C, normal diet (n = 8); SH, sham+HFD (n = 10); OH, OVX+HFD (n = 8); OVX, ovariectomy; HFD, high fat diet.

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