Involucrin Antibody - #AF0186

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製品: Involucrin Antibody
カタログ: AF0186
タンパク質の説明: Rabbit polyclonal antibody to Involucrin
アプリケーション: WB IHC IF/ICC
Cited expt.: IHC
反応性: Human, Mouse
分子量: 68~120kDa; 68kD(Calculated).
ユニプロット: P07476
RRID: AB_2833379

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
Involucrin Antibody detects endogenous levels of total Involucrin.
RRID:
AB_2833379
引用形式: Affinity Biosciences Cat# AF0186, RRID:AB_2833379.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

INVO_HUMAN; Involucrin; IVL;

免疫原

免疫原:

A synthesized peptide derived from human Involucrin

Uniprot:

P07476(INVO_HUMAN) >>Visit HPA database.

遺伝子(ID):
IVL(3713)
発現特異性:
P07476 INVO_HUMAN:

Keratinocytes of epidermis and other stratified squamous epithelia.

タンパク質の説明:
IVL Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the involucrin family. Directly or indirectly cross-linked to cornifelin (CNFN).
タンパク質配列:
MSQQHTLPVTLSPALSQELLKTVPPPVNTHQEQMKQPTPLPPPCQKVPVELPVEVPSKQEEKHMTAVKGLPEQECEQQQKEPQEQELQQQHWEQHEEYQKAENPEQQLKQEKTQRDQQLNKQLEEEKKLLDQQLDQELVKRDEQLGMKKEQLLELPEQQEGHLKHLEQQEGQLKHPEQQEGQLELPEQQEGQLELPEQQEGQLELPEQQEGQLELPEQQEGQLELPEQQEGQLELPQQQEGQLELSEQQEGQLELSEQQEGQLKHLEHQEGQLEVPEEQMGQLKYLEQQEGQLKHLDQQEKQPELPEQQMGQLKHLEQQEGQPKHLEQQEGQLEQLEEQEGQLKHLEQQEGQLEHLEHQEGQLGLPEQQVLQLKQLEKQQGQPKHLEEEEGQLKHLVQQEGQLKHLVQQEGQLEQQERQVEHLEQQVGQLKHLEEQEGQLKHLEQQQGQLEVPEQQVGQPKNLEQEEKQLELPEQQEGQVKHLEKQEAQLELPEQQVGQPKHLEQQEKHLEHPEQQDGQLKHLEQQEGQLKDLEQQKGQLEQPVFAPAPGQVQDIQPALPTKGEVLLPVEHQQQKQEVQWPPKHK

研究背景

機能:

Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

PTMs:

Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.

細胞の位置付け:

Cytoplasm.
Note: Constituent of the scaffolding of the cornified envelope.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Keratinocytes of epidermis and other stratified squamous epithelia.

タンパク質ファミリー:

Belongs to the involucrin family.

参考文献

1). The purinergic receptor P2rx7 mediated ATP sensing is required to prevent bone aging by directing mitochondrial fitness of MSCs. Journal of advanced research, 2025 (PubMed: 40518112) [IF=11.4]
2). Use of mouse primary epidermal organoids for USA300 infection modeling and drug screening. Research Square, 2022 (PubMed: 36631452) [IF=8.1]

Application: IHC    Species: Mouse    Sample: mPEOs

Fig. 1: Characterization of mouse primary epidermal organoids (mPEOs).A Overview of mPEOs formed from single cells within 7 d (scale bars, 100 μm). B Increased number of mPEOs observed within 7 d in bright field (scale bars, 100 μm). C H&E staining of mPEOs cultured for 7 d (scale bars, 50 μm). D Ultrathin sections in TEM images of mPEOs (scale bars, 20 μm), SB stratum basale, SP stratum spinosum, GR stratum granulosum, KG keratohyalin granules, SC stratum corneum. E Immunofluorescence staining analysis of various skin-associated specific biomarkers in mPEOs (scale bars, 200 μm). F Immunohistochemical staining of paraffin-embedded mPEOs. Representative images of Loricrin, KRT10, Involucrin, KRT14 and Ki67 immunoreactivity in organoids (scale bars, 50 μm).
3). GDU-952, a novel AhR agonist ameliorates skin barrier abnormalities and immune dysfunction in DNFB-induced atopic dermatitis in mice. Biochemical pharmacology, 2023 (PubMed: 37778446) [IF=5.3]
4). A multi-target combinatorial therapy of MDBA alleviates atopic dermatitis via synchronized immunosuppression and barrier repair. European journal of pharmacology, 2026 (PubMed: 41802486) [IF=4.2]

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