Calcitonin Antibody - #DF8512

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製品: Calcitonin Antibody
カタログ: DF8512
タンパク質の説明: Rabbit polyclonal antibody to Calcitonin
アプリケーション: WB IHC IF/ICC
Cited expt.: IHC
反応性: Human, Mouse, Rat
予測: Dog, Chicken
分子量: 13 kDa; 14kD,15kD(Calculated).
ユニプロット: P06881 | P01258
RRID: AB_2841717

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
Calcitonin Antibody detects endogenous levels of total Calcitonin.
RRID:
AB_2841717
引用形式: Affinity Biosciences Cat# DF8512, RRID:AB_2841717.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Calcitonin/calcitonin related polypeptide alpha; Alpha type CGRP; Beta type CGRP; CALC1; CALC2; CALCA; CALCB; Calcitonin 1; Calcitonin 2; Calcitonin gene related peptide I; Calcitonin gene related peptide II; Calcitonin related polypeptide alpha; Calcitonin related polypeptide beta; CGRP; CGRP I; CGRP II; CGRP1; CGRP2; CT; Katacalcin; KC; Alpha CGRP; alpha type CGRP; CALC 1; CALC A; CALC1; calcitonin 1; calcitonin gene related peptide 1; calcitonin gene related peptide I; calcitonin related polypeptide alpha; CGRP 1; CGRP; CGRP I; CGRP1; CT; katacalcin; KC;

免疫原

免疫原:

A synthesized peptide derived from human Calcitonin, corresponding to a region within the internal amino acids.

Uniprot:

P06881(CALCA_HUMAN) >>Visit HPA database.

P01258(CALC_HUMAN) >>Visit HPA database.

遺伝子(ID):
CALCA(796)
タンパク質配列:
MGFQKFSPFLALSILVLLQAGSLHAAPFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSRIIAQKRACDTATCVTHRLAGLLSRSGGVVKNNFVPTNVGSKAFGRRRRDLQA

MGFQKFSPFLALSILVLLQAGSLHAAPFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSSLDSPRSKRCGNLSTCMLGTYTQDFNKFHTFPQTAIGVGAPGKKRDMSSDLERDHRPHVSMPQNAN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Chicken
100
Pig
75
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the calcitonin family.

機能:

Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.

Katacalcin is a potent plasma calcium-lowering peptide.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the calcitonin family.

参考文献

1). Qizhi Tongluo capsule improves vascular dementia in rats via integrated metabolomics and molecular mechanism research. Journal of ethnopharmacology, 2026 (PubMed: 41167571) [IF=4.8]
2). Partially brain effects of injection of human umbilical cord mesenchymal stem cells at injury sites in a mouse model of thoracic spinal cord contusion. Frontiers in molecular neuroscience, 2023 (PubMed: 37342099) [IF=3.5]

Application: IHC    Species: Mouse    Sample:

Figure 5. (A–D) Immunohistochemistry was used to detect the expression of CGRP, Substance P, C-Fos and KCC2 in the anterior cingulate cortex (ACC). (E1–E4) The integrated optical density (IOD) was quantitatively analyzed (IOD/area) to determine marker expression. The expression of CGRP, Substance P and C-Fos in the ACC in the SCI model increased, and the expression of KCC2 decreased (one-way ANOVA; SCI vs. sham, **P < 0.0001). The expression of CGRP, Substance P and C-Fos decreased, whereas that of KCC2 increased, after local injection of HU-MSCs (one-way ANOVA; SCI + HU-MSC vs. SCI/SCI + PBS, **P < 0.0001).

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